DEVELOPMENT OF PCR-ELISA TECHNIQUE FOR DETERMINATION OF HLA DRB1*01 GROUP ALLELES

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NASIRI H.; FOROUZANDEH M.; RASAEI M.J.; POURFATH ELAH A.A.; RAHBARIZADEH F.

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Journal Paper

Paper Information

Journal: Iranian Journal of Biotechnology Year:2004 | Volume:2 | Issue:3 Page(s): 164-169

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Information Journal Paper

Title

DEVELOPMENT OF PCR-ELISA TECHNIQUE FOR DETERMINATION OF HLA DRB1*01 GROUP ALLELES

Author(s)

Keywords

HLA DRB1

PCR-ELISA

HYBRIDIZATION

GROUP SPECIFIC AMPLIFICATION

Abstract

We have developed an allotyping assay for detection of four HLA DRB1*01 group alleles based on polymerase chain reaction and solution HYBRIDIZATION in a microtiter plate. Using group specific primers a region within exon 2 of HLA DRB1 gene was amplified by PCR. Labeling of PCR product was achieved by adding small amount of Dig-dUTP in place of dTTP. Labeled PCR product was hybridized to allele (HLA DRB1*0101, *0102, *0103 and *0104) specific and a group (HLA DRB1*01) specific oligonucleotide probes in separate wells of the plate. Hybridized amplicones were detected by an enzymatic procedure. Ninety DNA samples were tested in parallel with PCR-SSP typing. The results were found to be well correlated by two methods. These results further suggest that, PCRELISA would be a rapid, specific and simple method that can be used for high resolution HLA typing before bone marrow and stem cell transplantation.

Cites

DETECTION OF HEAT-LABILE TOXIN IN ENTEROTOXIGENIC ESCHERICHIA COLI USING PCR-ELISA TECHNIQUE

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APA: Copy

NASIRI, H., FOROUZANDEH, M., RASAEI, M.J., POURFATH ELAH, A.A., & RAHBARIZADEH, F.. (2004). DEVELOPMENT OF PCR-ELISA TECHNIQUE FOR DETERMINATION OF HLA DRB1*01 GROUP ALLELES . IRANIAN JOURNAL OF BIOTECHNOLOGY, 2(3), 164-169. SID. https://sid.ir/paper/529375/en

Vancouver: Copy

NASIRI H., FOROUZANDEH M., RASAEI M.J., POURFATH ELAH A.A., RAHBARIZADEH F.. DEVELOPMENT OF PCR-ELISA TECHNIQUE FOR DETERMINATION OF HLA DRB1*01 GROUP ALLELES . IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2004;2(3):164-169. Available from: https://sid.ir/paper/529375/en

IEEE: Copy

H. NASIRI, M. FOROUZANDEH, M.J. RASAEI, A.A. POURFATH ELAH, and F. RAHBARIZADEH, “DEVELOPMENT OF PCR-ELISA TECHNIQUE FOR DETERMINATION OF HLA DRB1*01 GROUP ALLELES ,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 2, no. 3, pp. 164–169, 2004, [Online]. Available: https://sid.ir/paper/529375/en

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