DETECTION OF HEAT-LABILE TOXIN IN ENTEROTOXIGENIC ESCHERICHIA COLI USING PCR-ELISA TECHNIQUE

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ESFANDIARI P.; AMANI J.; IMANI FOOLADI A.A.; FORGHANIFARD M.M.; MIRHOSSEINI S.A.

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Journal: JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES Year:2015 | Volume:17 | Issue:3 (55) Page(s): 114-121

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Information Journal Paper

Title

DETECTION OF HEAT-LABILE TOXIN IN ENTEROTOXIGENIC ESCHERICHIA COLI USING PCR-ELISA TECHNIQUE

Author(s)

Keywords

ENTEROTOXIGENIC ESCHERICHIA COLIQ2

PCRQ2

ELISAQ2

HEATQ2

LABILE ENTEROTOXINQ2

Abstract

Background and Objective: ENTEROTOXIGENIC ESCHERICHIA COLI (ETEC) are the most common agent which causes diarrhea, worldwide. ETEC is colonized along the cells and then producing HEAT-labile (LT) and HEAT-stable enterotoxigenic which enter into intestinal epithelial cells and causes water and electrolyte loss from intestinal epithelial cells and eventually cause diarrhea.This study was done to detect the HEAT-labile toxin in ENTEROTOXIGENIC ESCHERICHIA COLI using PCR-ELISA technique.Methods: In this descriptive study, DIG-labeled PCR products were bounded to streptoavidin-coated wells of a microtiter plate and detected by anti-DIG–peroxidase conjugate. The biotin-labeled internal probe was used for verification of PCR products.Results: HEAT-labile toxin was detected by PCR-ELISA method. The sensitivity of HEAT-labile toxin was 1.9 ng. This method did not cross-react with bacteria from this variety.Conclusion: PCR-ELISA method is 100 times more sensitive than conventional PCR method and due to lack of agarose gel and electrophoresis device it can be a good alternative to traditional method.

Cites

Detection of Specific eae Gene from Enteropathogenic Escherichia coli by PCR-ELISA

References

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APA: Copy

ESFANDIARI, P., AMANI, J., IMANI FOOLADI, A.A., FORGHANIFARD, M.M., & MIRHOSSEINI, S.A.. (2015). DETECTION OF HEAT-LABILE TOXIN IN ENTEROTOXIGENIC ESCHERICHIA COLI USING PCR-ELISA TECHNIQUE. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, 17(3 (55)), 114-121. SID. https://sid.ir/paper/79481/en

Vancouver: Copy

ESFANDIARI P., AMANI J., IMANI FOOLADI A.A., FORGHANIFARD M.M., MIRHOSSEINI S.A.. DETECTION OF HEAT-LABILE TOXIN IN ENTEROTOXIGENIC ESCHERICHIA COLI USING PCR-ELISA TECHNIQUE. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2015;17(3 (55)):114-121. Available from: https://sid.ir/paper/79481/en

IEEE: Copy

P. ESFANDIARI, J. AMANI, A.A. IMANI FOOLADI, M.M. FORGHANIFARD, and S.A. MIRHOSSEINI, “DETECTION OF HEAT-LABILE TOXIN IN ENTEROTOXIGENIC ESCHERICHIA COLI USING PCR-ELISA TECHNIQUE,” JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, vol. 17, no. 3 (55), pp. 114–121, 2015, [Online]. Available: https://sid.ir/paper/79481/en

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