FED-BATCH CULTIVATION OF RECOMBINANT ESCHERICHIA COLI PRODUCING HUMAN INTERFERON-G UNDER CONTROLLED SPECIFIC GROWTH RATE

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KHALILZADEH R.; SHOJA ALSADATI S.A.; BAHRAMI A.; MAGHSOUDI N.

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Journal Paper

Paper Information

Journal: Iranian Journal of Biotechnology Year:2004 | Volume:2 | Issue:2 Page(s): 113-122

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Information Journal Paper

Title

FED-BATCH CULTIVATION OF RECOMBINANT ESCHERICHIA COLI PRODUCING HUMAN INTERFERON-G UNDER CONTROLLED SPECIFIC GROWTH RATE

Author(s)

KHALILZADEH R. | SHOJA ALSADATI S.A. | BAHRAMI A. | MAGHSOUDI N. | Issue Writer Certificate

Keywords

HUMAN INTERFERON-GAMMA

FED-BATCH CULTIVATION

RECOMBINANT E. COLI

SPECIFIC GROWTH RATE

GLUCOSE

GLYCEROL

Abstract

A simple fed-batch process with pre-determined exponential feeding strategy for high-cell-density cultivation of RECOMBINANT E. COLI BL21 (DE3) in defined medium was developed. In this feeding method GLUCOSE and GLYCEROL were used as the sole sources of carbon and energy to increase the cell density exponentially at controlled SPECIFIC GROWTH RATEs, which do not cause the accumulation of acetate. Thus, sophisticated feedback control or extra equipment to prevent the accumulation of toxic level of acetate is not necessary. The final cell densities of 100 and 118 gl-1 of dry cell mass for RECOMBINANT E. COLI producing human interferon-g (hIFN-g) were obtained by using GLUCOSE and GLYCEROL, respectively. The concentration of acetate was always maintained below toxic level. The specific yield of hIFN-g with GLUCOSE and GLYCEROL was 93 and 92 mgg-1 of dry cell mass, and the overall productivity of hIFN-g was 0.16 and 0.14 gl-1h-1 for these two carbon sources, respectively.

Cites

OPTIMIZATION OF CULTURAL CONDITION TO IMPROVE RECOMBINANT C-D FRAGMENT OF BACTERIORHODOPSIN PRODUCTION IN E. COLI

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APA: Copy

KHALILZADEH, R., SHOJA ALSADATI, S.A., BAHRAMI, A., & MAGHSOUDI, N.. (2004). FED-BATCH CULTIVATION OF RECOMBINANT ESCHERICHIA COLI PRODUCING HUMAN INTERFERON-G UNDER CONTROLLED SPECIFIC GROWTH RATE. IRANIAN JOURNAL OF BIOTECHNOLOGY, 2(2), 113-122. SID. https://sid.ir/paper/531425/en

Vancouver: Copy

KHALILZADEH R., SHOJA ALSADATI S.A., BAHRAMI A., MAGHSOUDI N.. FED-BATCH CULTIVATION OF RECOMBINANT ESCHERICHIA COLI PRODUCING HUMAN INTERFERON-G UNDER CONTROLLED SPECIFIC GROWTH RATE. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2004;2(2):113-122. Available from: https://sid.ir/paper/531425/en

IEEE: Copy

R. KHALILZADEH, S.A. SHOJA ALSADATI, A. BAHRAMI, and N. MAGHSOUDI, “FED-BATCH CULTIVATION OF RECOMBINANT ESCHERICHIA COLI PRODUCING HUMAN INTERFERON-G UNDER CONTROLLED SPECIFIC GROWTH RATE,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 2, no. 2, pp. 113–122, 2004, [Online]. Available: https://sid.ir/paper/531425/en

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