THE EXPRESSION OF HUMAN GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR BY HEAT-INDUCTION IN ESCHERICHIA COLI

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GHANBARIAN H.; ZOMORODIPOUR A.; ATAEI FARNAZ; SHOJAI S.; YAKHCHALI B.

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Journal: Journal of Sciences, Islamic Republic of Iran Year:2004 | Volume:15 | Issue:3 Page(s): 203-210

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Title

THE EXPRESSION OF HUMAN GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR BY HEAT-INDUCTION IN ESCHERICHIA COLI

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Keywords

RECOMBINANT HUMAN GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR (RHGM-CSF); LAMBDA (X) PL; HEAT SHOCK-INDUCTION

Abstract

A self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ëpL promoter, was constructed. The designed 5024 base pair expression plasmid contained a heat sensitive repressor cI857 coding gene to regulate the function of ëpL promoter under heat shock induction. Using the constructed vector, for the first time, periplasmic production of human granulocyte macrophage colony stimulating factor (hGM-CSF) under heat induction was demonstrated. The developed hGM-CSF expressing system in this study has potential for the secretion of nearly 100% of expressed proteins into the Escherichia coli periplasmic space upon temperature up-shift.

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APA: Copy

GHANBARIAN, H., ZOMORODIPOUR, A., ATAEI, FARNAZ, SHOJAI, S., & YAKHCHALI, B.. (2004). THE EXPRESSION OF HUMAN GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR BY HEAT-INDUCTION IN ESCHERICHIA COLI. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, 15(3), 203-210. SID. https://sid.ir/paper/531453/en

Vancouver: Copy

GHANBARIAN H., ZOMORODIPOUR A., ATAEI FARNAZ, SHOJAI S., YAKHCHALI B.. THE EXPRESSION OF HUMAN GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR BY HEAT-INDUCTION IN ESCHERICHIA COLI. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN[Internet]. 2004;15(3):203-210. Available from: https://sid.ir/paper/531453/en

IEEE: Copy

H. GHANBARIAN, A. ZOMORODIPOUR, FARNAZ ATAEI, S. SHOJAI, and B. YAKHCHALI, “THE EXPRESSION OF HUMAN GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR BY HEAT-INDUCTION IN ESCHERICHIA COLI,” JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, vol. 15, no. 3, pp. 203–210, 2004, [Online]. Available: https://sid.ir/paper/531453/en

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