FREQUENCY OF BOVINE LYMPHOCYTE ANTIGEN DRB3.2 ALLELES IN SARABI COWS

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MONTAZER TORBATI F.; EFTEKHARI SHAHROUDI F.; NASIRI M.R.; SAFARNEZHAD ABAS; MONTAZER TORBATI M.B.

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Journal Paper

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Journal: Iranian Journal of Biotechnology Year:2004 | Volume:2 | Issue:2 Page(s): 101-105

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Title

FREQUENCY OF BOVINE LYMPHOCYTE ANTIGEN DRB3.2 ALLELES IN SARABI COWS

Author(s)

Keywords

BOLA-DRB3.2

POLYMERASE CHAIN REACTION (PCR)

RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP)

IRANIAN SARABI COWS.

Abstract

The second exon of the bovine Major Histocompatibility Complex (MHC) class II DRB3 gene was amplified by polymerase chain reaction (PCR) from 50 DNA samples of Iranian Sarabi cattle. Bovine DNA was isolated from aliquots of whole blood. A two-step polymerase chain reaction followed by digestion with restriction endonucleases RsaI, BstyI, and HaeIII was conducted on DNA from samples. Fifteen Bovine Lymphocyte Antigen (BoLA)-DRB3 alleles were assigned, including some that were only recently described for zebu cattle. Allelic frequencies ranged from 0.02 to 0.23. The most frequent alleles were *52 (frequency = 0.23), *11 (0.18) and *23 (0.15). Results of this study demonstrate that the BOLA-DRB3.2 locus is highly polymorphic in Sarabi cattles.

Cites

POLYMORPHISM OF BOVINE LYMPHOCYTE ANTIGEN DRB3.2 IN HOLSTEIN BULLS OF IRAN USING PCR-RFLP

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APA: Copy

MONTAZER TORBATI, F., EFTEKHARI SHAHROUDI, F., NASIRI, M.R., SAFARNEZHAD, ABAS, & MONTAZER TORBATI, M.B.. (2004). FREQUENCY OF BOVINE LYMPHOCYTE ANTIGEN DRB3.2 ALLELES IN SARABI COWS. IRANIAN JOURNAL OF BIOTECHNOLOGY, 2(2), 101-105. SID. https://sid.ir/paper/532198/en

Vancouver: Copy

MONTAZER TORBATI F., EFTEKHARI SHAHROUDI F., NASIRI M.R., SAFARNEZHAD ABAS, MONTAZER TORBATI M.B.. FREQUENCY OF BOVINE LYMPHOCYTE ANTIGEN DRB3.2 ALLELES IN SARABI COWS. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2004;2(2):101-105. Available from: https://sid.ir/paper/532198/en

IEEE: Copy

F. MONTAZER TORBATI, F. EFTEKHARI SHAHROUDI, M.R. NASIRI, ABAS SAFARNEZHAD, and M.B. MONTAZER TORBATI, “FREQUENCY OF BOVINE LYMPHOCYTE ANTIGEN DRB3.2 ALLELES IN SARABI COWS,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 2, no. 2, pp. 101–105, 2004, [Online]. Available: https://sid.ir/paper/532198/en

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