مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

DETECTION OF BOVINE VIRAL DIARRHEA VIRUS IN BOVINE SEMEN USING NESTED-PCR

Pages

  48-51

Abstract

 A rapid and sensitive reverse transcription polymerase chain reaction (RT-PCR) and NESTED-PCR were used to detect BOVINE VIRAL DIARRHEA VIRUS 1 (BVDV-1) in bull SEMEN. Selected primers could amplify a part of the 5´UTR of the BVDV genome. A 294 bp DNA fragment was amplified and specificity of the results was confirmed by direct sequencing of the PCR product. Prior to RNA extraction, the seminal inhibitors were eliminated using a simple dilution method. Therefore, a sensitivity of 3×102 50% tissue culture infective dose (TCID50) was achieved when experimentally infected SEMEN was used for RNA extraction. In NESTED-PCR a 160 bp fragment was amplified and sensitivity of the test was increased to 3 TCID50. This technique can be used as a rapid and sensitive method of BVDV-1 detection in bovine SEMEN.

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