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Information Journal Paper

Title

DETECTION OF BOVINE VIRAL DIARRHEA VIRUS IN BOVINE SEMEN USING NESTED-PCR

Pages

  48-51

Abstract

 A rapid and sensitive reverse transcription polymerase chain reaction (RT-PCR) and NESTED-PCR were used to detect BOVINE VIRAL DIARRHEA VIRUS 1 (BVDV-1) in bull SEMEN. Selected primers could amplify a part of the 5´UTR of the BVDV genome. A 294 bp DNA fragment was amplified and specificity of the results was confirmed by direct sequencing of the PCR product. Prior to RNA extraction, the seminal inhibitors were eliminated using a simple dilution method. Therefore, a sensitivity of 3×102 50% tissue culture infective dose (TCID50) was achieved when experimentally infected SEMEN was used for RNA extraction. In NESTED-PCR a 160 bp fragment was amplified and sensitivity of the test was increased to 3 TCID50. This technique can be used as a rapid and sensitive method of BVDV-1 detection in bovine SEMEN.

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    Cite

    APA: Copy

    DALIRI, M., MORSHEDI, D., HAJIAN, T., GHOREYSHI, S.A., & AFSHAR, K.. (2007). DETECTION OF BOVINE VIRAL DIARRHEA VIRUS IN BOVINE SEMEN USING NESTED-PCR. IRANIAN JOURNAL OF BIOTECHNOLOGY, 5(1), 48-51. SID. https://sid.ir/paper/540161/en

    Vancouver: Copy

    DALIRI M., MORSHEDI D., HAJIAN T., GHOREYSHI S.A., AFSHAR K.. DETECTION OF BOVINE VIRAL DIARRHEA VIRUS IN BOVINE SEMEN USING NESTED-PCR. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2007;5(1):48-51. Available from: https://sid.ir/paper/540161/en

    IEEE: Copy

    M. DALIRI, D. MORSHEDI, T. HAJIAN, S.A. GHOREYSHI, and K. AFSHAR, “DETECTION OF BOVINE VIRAL DIARRHEA VIRUS IN BOVINE SEMEN USING NESTED-PCR,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 5, no. 1, pp. 48–51, 2007, [Online]. Available: https://sid.ir/paper/540161/en

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