USING RECOMBINANT CHLAMYDIA MAJOR OUTER MEMBRANE PROTEIN (MOMP) IN ELISA DIAGNOSTIC KIT

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BANDEHPOUR M.; SEYED N.; SHAD NOUSH M.; PAKZAD P.; KAZEMI B.

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Journal Paper

Paper Information

Journal: Iranian Journal of Biotechnology Year:2006 | Volume:4 | Issue:4 Page(s): 239-244

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Information Journal Paper

Title

USING RECOMBINANT CHLAMYDIA MAJOR OUTER MEMBRANE PROTEIN (MOMP) IN ELISA DIAGNOSTIC KIT

Author(s)

Keywords

CHLAMYDIA TRACHOMATIS

RECOMBINANT MOMP PROTEIN

EXPRESSION

Abstract

CHLAMYDIA TRACHOMATIS is one of the main causes of Sexually Transmitted Diseases (STDs) such as prostatitis and epididymitis in men and cervicitis, endometriosis, vaginitis and ureogenital tract infections in women. Serological tests with sensitivities related to specific antigens are commonly used as routine laboratory tests for diagnosis of Chlamydia. In this research the Chlamydia Major Outer Membrane Protein gene was coloned in order to prepare a specific recombinant protein for use in the ELISA diagnostic kit. DNA was extracted from cultured C. tachomatis. PCR reaction was carried out and the resulting PCR product was cloned into the pGemex-1 EXPRESSION vector and induced by IPTG (Isopropyl β-DThiogalactopyrano side). Recombinant protein was confirmed by gel diffusion, dot blot and western blot, using patient’s serum. The use of recombinant protein for diagnosis of Chlamydia by ELISA is therefore recommended.

Cites

HIGH-LEVEL EXPRESSION OF IMMUNOGENIC RECOMBINANT PLASMO-DIUM VIVAX MEROZOITE SURFACE PROTEIN (PVMSP-142 KDA) IN PGEX 6P1 VECTOR

References

EVALUATION OF CHLAMYDIA PNEUMONIAE 43- AND 53-KILODALTON RECOMBINANT PROTEINS FOR SERODIAGNOSIS BY WESTERN BLOT

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APA: Copy

BANDEHPOUR, M., SEYED, N., SHAD NOUSH, M., PAKZAD, P., & KAZEMI, B.. (2006). USING RECOMBINANT CHLAMYDIA MAJOR OUTER MEMBRANE PROTEIN (MOMP) IN ELISA DIAGNOSTIC KIT. IRANIAN JOURNAL OF BIOTECHNOLOGY, 4(4), 239-244. SID. https://sid.ir/paper/547269/en

Vancouver: Copy

BANDEHPOUR M., SEYED N., SHAD NOUSH M., PAKZAD P., KAZEMI B.. USING RECOMBINANT CHLAMYDIA MAJOR OUTER MEMBRANE PROTEIN (MOMP) IN ELISA DIAGNOSTIC KIT. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2006;4(4):239-244. Available from: https://sid.ir/paper/547269/en

IEEE: Copy

M. BANDEHPOUR, N. SEYED, M. SHAD NOUSH, P. PAKZAD, and B. KAZEMI, “USING RECOMBINANT CHLAMYDIA MAJOR OUTER MEMBRANE PROTEIN (MOMP) IN ELISA DIAGNOSTIC KIT,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 4, no. 4, pp. 239–244, 2006, [Online]. Available: https://sid.ir/paper/547269/en

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