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Information Journal Paper

Title

DETERMINATION OF JUGLONE (5-HYDROXY 1,4-NAPHTHOQUINONE) IN PTEROCARYA FRAXINIFOLIA BY RP-HPLC

Pages

  73-76

Abstract

 A method for determination of JUGLONE in leaves and hulls of PTEROCARYA FRAXINIFOLIA was developed based on RP-HPLC. This compound was extracted from leaves and hulls by chloroform refluxing about 2 hours. The chloroform was evaporated under low pressure at   50 ºC to produce a dry residue. The remained materials were dissolved in acetonitrile to be used for determination of this compound. Appropriate conditions for RP-HPLC were determined using standard of JUGLONE. These conditions include, acetonitrile percentage in mobile phase, pH of mobile phase (phosphate buffer) and temperature of column, which were 50%, 4 and 30ºC, respectively. JUGLONE concentration was determined using standard addition method. The concentrations of JUGLONE were 2.15, 2.74,1.77,1.12and 0.34 g in 100 g of dry leaves in May, June, July, August and September 2001, respectively. The content of JUGLONE in hulls was 0.44 g in 100 g of dry hulls in May.

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  • Cite

    APA: Copy

    HAJ MOHAMMADI, M.R., & KAMEL, K.. (2006). DETERMINATION OF JUGLONE (5-HYDROXY 1,4-NAPHTHOQUINONE) IN PTEROCARYA FRAXINIFOLIA BY RP-HPLC. IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE), 25(4), 73-76. SID. https://sid.ir/paper/550593/en

    Vancouver: Copy

    HAJ MOHAMMADI M.R., KAMEL K.. DETERMINATION OF JUGLONE (5-HYDROXY 1,4-NAPHTHOQUINONE) IN PTEROCARYA FRAXINIFOLIA BY RP-HPLC. IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE)[Internet]. 2006;25(4):73-76. Available from: https://sid.ir/paper/550593/en

    IEEE: Copy

    M.R. HAJ MOHAMMADI, and K. KAMEL, “DETERMINATION OF JUGLONE (5-HYDROXY 1,4-NAPHTHOQUINONE) IN PTEROCARYA FRAXINIFOLIA BY RP-HPLC,” IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE), vol. 25, no. 4, pp. 73–76, 2006, [Online]. Available: https://sid.ir/paper/550593/en

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