EXPRESSION OF RECOMBINANT ALPHA-1 ANTITRYPSIN IN CHO AND COS-7 CELL LINES USING LENTIVIRAL VECTOR

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GHAEDI M.; SAHEBGHADAM LOTFI A.; SOLEYMANI MASOUD; SHAMSARA M.; ARJMAND S.; ADIBI B.

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF BIOTECHNOLOGY Year:2009 | Volume:7 | Issue:3 Page(s): 148-156

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Title

EXPRESSION OF RECOMBINANT ALPHA-1 ANTITRYPSIN IN CHO AND COS-7 CELL LINES USING LENTIVIRAL VECTOR

Author(s)

Keywords

ALPHA-1 ANTITRYPSIN

TRANSFECTION

PROTEIN PRODUCTION

LENTIVIRAL VECTOR

Abstract

In this study, in order to facilitate and accelerate the production of eukaryotic protein alpha 1-antitrypsin (AAT) with correct post-translational modifications, a protein production system based on the transduction of CHO and COS-7 cells using lentiviral vectors was developed. Human AAT Cdna was cloned into a replication- defective lentiviral vector. The transgene AAT-Jred chimer was transferred to CHO and COS-7 cell lines using this vector and its expressions were visualized by fluorescent microscopy. The mRNA expression levels of the AAT genes were determined using Revearse Transcriptase-Polymerase Chain Reaction (RT-PCR) and its secretion into the medium by both cell types was determined using ELISA. The results show that by employing a lentiviral vector, efficient genetic loading of CHO and COS-7 cells with the AAT gene was achieved. In conclusion, by using aLentivirus-based gene delivery system, large amounts of recombinant human AAT protein were expressed in both CHO and COS-7 cell lines. This expression system possesses key properties that ensure its application in the delivery of therapeutic genes into mammalian cultured cells.

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APA: Copy

GHAEDI, M., SAHEBGHADAM LOTFI, A., SOLEYMANI, MASOUD, SHAMSARA, M., ARJMAND, S., & ADIBI, B.. (2009). EXPRESSION OF RECOMBINANT ALPHA-1 ANTITRYPSIN IN CHO AND COS-7 CELL LINES USING LENTIVIRAL VECTOR. IRANIAN JOURNAL OF BIOTECHNOLOGY, 7(3), 148-156. SID. https://sid.ir/paper/558409/en

Vancouver: Copy

GHAEDI M., SAHEBGHADAM LOTFI A., SOLEYMANI MASOUD, SHAMSARA M., ARJMAND S., ADIBI B.. EXPRESSION OF RECOMBINANT ALPHA-1 ANTITRYPSIN IN CHO AND COS-7 CELL LINES USING LENTIVIRAL VECTOR. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2009;7(3):148-156. Available from: https://sid.ir/paper/558409/en

IEEE: Copy

M. GHAEDI, A. SAHEBGHADAM LOTFI, MASOUD SOLEYMANI, M. SHAMSARA, S. ARJMAND, and B. ADIBI, “EXPRESSION OF RECOMBINANT ALPHA-1 ANTITRYPSIN IN CHO AND COS-7 CELL LINES USING LENTIVIRAL VECTOR,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 7, no. 3, pp. 148–156, 2009, [Online]. Available: https://sid.ir/paper/558409/en

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