EXPRESSION OF THE RECOMBINANT PML PROTEIN UNDER THE INDUCIBLI VIRAL T5 PROMOTER

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VALIAN BOROOJENI M.S.

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Journal: RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE" Year:2003 | Volume:17 | Issue:1 Page(s): 103-120

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Title

EXPRESSION OF THE RECOMBINANT PML PROTEIN UNDER THE INDUCIBLI VIRAL T5 PROMOTER

Author(s)

VALIAN BOROOJENI M.S. | Issue Writer Certificate

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Abstract

In this study, the expression of the recombinant promyelocytic leukemia (PM protein under the T5 promoter has been studied. To express the PML protein, its complementary DNA (cDNA) was specifically amplified using polymerase ch reaction, PCR, and cloned into pQE-30 vector under the T5 promoter. The result recombinant vector was then introduced into BL21 bacterial host cells, and expression was induced. The expressed proteins were then purified from the to: cell extract by means of the nickel affinity chromatography. The specific express of the PML protein was analyzed using SDS-PAGE electrophoresis with Coomas blue staining and immunoblotting with anti-Histidine antibody. The results show that the PML protein was specifically expressed in a large quantity. It is, there is concluded that the T5 promoter can provide a suitable system for

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APA: Copy

VALIAN BOROOJENI, M.S.. (2003). EXPRESSION OF THE RECOMBINANT PML PROTEIN UNDER THE INDUCIBLI VIRAL T5 PROMOTER. RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE", 17(1), 103-120. SID. https://sid.ir/paper/55862/en

Vancouver: Copy

VALIAN BOROOJENI M.S.. EXPRESSION OF THE RECOMBINANT PML PROTEIN UNDER THE INDUCIBLI VIRAL T5 PROMOTER. RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE"[Internet]. 2003;17(1):103-120. Available from: https://sid.ir/paper/55862/en

IEEE: Copy

M.S. VALIAN BOROOJENI, “EXPRESSION OF THE RECOMBINANT PML PROTEIN UNDER THE INDUCIBLI VIRAL T5 PROMOTER,” RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE", vol. 17, no. 1, pp. 103–120, 2003, [Online]. Available: https://sid.ir/paper/55862/en

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