SHORT COMMUNICATION: OPTIMIZATION OF SECRETORY EXPRESSION OF RECOMBINANT HGM-CSF IN HIGH CELL DENSITY CULTIVATION OF RECOMBINANT ESCHERICHIA COLI USING TAGUCHI STATISTICAL METHOD

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HAJINIA ELMIRA; FATEMI SEYED SAFA ALI; KARKHANEH ALI ASGHAR; SAFEKORDI ALI AKBAR; YAKHCHALI BAGHER

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Journal: Iranian Journal of Biotechnology Year:2012 | Volume:10 | Issue:4 Page(s): 275-280

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Information Journal Paper

Title

SHORT COMMUNICATION: OPTIMIZATION OF SECRETORY EXPRESSION OF RECOMBINANT HGM-CSF IN HIGH CELL DENSITY CULTIVATION OF RECOMBINANT ESCHERICHIA COLI USING TAGUCHI STATISTICAL METHOD

Author(s)

Keywords

ESCHERICHIA COLI

FED-BATCH

HIGH CELL DENSITY CULTIVATION

HUMAN GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR

OPTIMIZATION

PURIFICATION

Abstract

Human granulocyte macrophage colony stimulating factor (hGM-CSF) has many therapeutic applications.In this study, in order to verify the PURIFICATION process, the effect of carbon source, IPTG concentration and post-induction time on the secretion of recombinant hGM-CSF into the culture medium by recombinant ESCHERICHIA COLI during HIGH CELL DENSITY CULTIVATION were evaluated by using the Taguchi statistical method. The results indicated that glucose, 1mM IPTG and a time of 6 h post-induction, represented optimum conditions. The secreted hGM-CSF, overall volumetric productivity and purified hGM-CSF were 373 mg/l, 18 mg/l/h and 63 mg/l, respectively.

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APA: Copy

HAJINIA, ELMIRA, FATEMI, SEYED SAFA ALI, KARKHANEH, ALI ASGHAR, SAFEKORDI, ALI AKBAR, & YAKHCHALI, BAGHER. (2012). SHORT COMMUNICATION: OPTIMIZATION OF SECRETORY EXPRESSION OF RECOMBINANT HGM-CSF IN HIGH CELL DENSITY CULTIVATION OF RECOMBINANT ESCHERICHIA COLI USING TAGUCHI STATISTICAL METHOD. IRANIAN JOURNAL OF BIOTECHNOLOGY, 10(4), 275-280. SID. https://sid.ir/paper/593048/en

Vancouver: Copy

HAJINIA ELMIRA, FATEMI SEYED SAFA ALI, KARKHANEH ALI ASGHAR, SAFEKORDI ALI AKBAR, YAKHCHALI BAGHER. SHORT COMMUNICATION: OPTIMIZATION OF SECRETORY EXPRESSION OF RECOMBINANT HGM-CSF IN HIGH CELL DENSITY CULTIVATION OF RECOMBINANT ESCHERICHIA COLI USING TAGUCHI STATISTICAL METHOD. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2012;10(4):275-280. Available from: https://sid.ir/paper/593048/en

IEEE: Copy

ELMIRA HAJINIA, SEYED SAFA ALI FATEMI, ALI ASGHAR KARKHANEH, ALI AKBAR SAFEKORDI, and BAGHER YAKHCHALI, “SHORT COMMUNICATION: OPTIMIZATION OF SECRETORY EXPRESSION OF RECOMBINANT HGM-CSF IN HIGH CELL DENSITY CULTIVATION OF RECOMBINANT ESCHERICHIA COLI USING TAGUCHI STATISTICAL METHOD,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 10, no. 4, pp. 275–280, 2012, [Online]. Available: https://sid.ir/paper/593048/en

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