PECTINASE ACTIVITY OF SOME MICROMYCETES ISOLATED FROM AGRICULTURAL SOILS

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NAYEBYAZDI N.; TAJICK GHANBARY M.A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Sciences, Islamic Republic of Iran Year:2012 | Volume:23 | Issue:4 Page(s): 305-311

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Title

PECTINASE ACTIVITY OF SOME MICROMYCETES ISOLATED FROM AGRICULTURAL SOILS

Author(s)

NAYEBYAZDI N. | TAJICK GHANBARY M.A. | Issue Writer Certificate

Keywords

ENZYME ACTIVITY

PECTINASE

FUNGAL SCREENING

IN VITRO

Abstract

Pectinases have multiple nature and various forms which are necessary for the hydrolysis of pectin in several conventional industrial and natural processes. In this investigation fourteen fungal strains which isolated from agricultural soils, were screened for PECTINASE activity. PECTINASE activity were measured IN VITRO by assaying released reducing sugar from pectin as sole carbon source.Aspergillus foetidusand Aspergillus aculeatus showed highest PECTINASE activity. Rhizoctonia solani (AG-4) and Trichoderma reesei S578 showed the highest level of protein production. Reducing sugars concentration were different between 13th and 15th then reduced to 29th days after inoculation. Secreted proteins increased to 11th and 13th then decreased to 29th days. Optimum pH and temprature for PECTINASE activity were 5 and 50oC respectively. Glucose was detected as a degradation product by thin layer chromatography (TLC). Electrophoresis of the extracellular proteins of A. foetidus and A. aculeatus revealed two major proteins having a molecular mass of 79 and 63 kDa respectively.

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APA: Copy

NAYEBYAZDI, N., & TAJICK GHANBARY, M.A.. (2012). PECTINASE ACTIVITY OF SOME MICROMYCETES ISOLATED FROM AGRICULTURAL SOILS. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, 23(4), 305-311. SID. https://sid.ir/paper/610293/en

Vancouver: Copy

NAYEBYAZDI N., TAJICK GHANBARY M.A.. PECTINASE ACTIVITY OF SOME MICROMYCETES ISOLATED FROM AGRICULTURAL SOILS. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN[Internet]. 2012;23(4):305-311. Available from: https://sid.ir/paper/610293/en

IEEE: Copy

N. NAYEBYAZDI, and M.A. TAJICK GHANBARY, “PECTINASE ACTIVITY OF SOME MICROMYCETES ISOLATED FROM AGRICULTURAL SOILS,” JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, vol. 23, no. 4, pp. 305–311, 2012, [Online]. Available: https://sid.ir/paper/610293/en

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