DEVELOPMENT OF RADIOLABELED RECOMBINANT ERYTHROPOIETIN FOR RECEPTOR STUDIES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

JALILIAN A.R.; SADEGHPOUR H.; AKHLAGHI M.; GHOLAMI E.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF NUCLEAR SCIENCE AND TECHNOLOGY Year:2012 | Volume:- | Issue:3 (61) Page(s): 73-79

Download Full-Text

View:

354

Download:

115

Cites:

Information Journal Paper

Title

DEVELOPMENT OF RADIOLABELED RECOMBINANT ERYTHROPOIETIN FOR RECEPTOR STUDIES

Author(s)

JALILIAN A.R. | SADEGHPOUR H. | AKHLAGHI M. | GHOLAMI E. | Issue Writer Certificate

Keywords

RADIOGALLIUM

EPO

RADIOLABELING

BIODISTRIBUTION

RADIOTRACER

Abstract

The study of human erythropoietin receptor is of great importance in evolution, carcinogenesis as well as endocrine research. In this study the production and quality control of human recombinant erythropoietin (EPO) has been reported. EPO was successively labeled with [67Ga]-gallium chloride after conjugation with freshly prepared cyclic DTPA-dianhydride (ccDTPA). The best results of the conjugation were obtained by the addition of 0.5 ml of a EPO pharmaceutical solution (in phosphate buffer, pH=8) to a glass tube pre-coated with DTPA-dianhydride (0.01 mg) at 25oC with continuous mild stirring for 30 min followed by HPLC/RTLC control and biological BIODISTRIBUTION in normal rats. Under optimized conditions, radio-thin layer chromatography (RTLC), instant thin layer chromatography (ITLC) and high performance liquid chromatography (HPLC) showed overall radiochemical purity of higher than 96% (specific activity=300-500 MBq/mg, labeling efficiency 77%). Preliminary in vivo studies in normal rat specimen demonstrated a high liver, lung spleen, kidney as well as long bones uptake of the tracer; the fact which is consistent with the reported EPO receptor sites. This tracer can be used in nuclear medicine and biological studies for evaluation of EPO in vitro and/or in vivo.

Cites

No record.

References

No record.

Cite

APA: Copy

JALILIAN, A.R., SADEGHPOUR, H., AKHLAGHI, M., & GHOLAMI, E.. (2012). DEVELOPMENT OF RADIOLABELED RECOMBINANT ERYTHROPOIETIN FOR RECEPTOR STUDIES. JOURNAL OF NUCLEAR SCIENCE AND TECHNOLOGY, -(3 (61)), 73-79. SID. https://sid.ir/paper/659030/en

Vancouver: Copy

JALILIAN A.R., SADEGHPOUR H., AKHLAGHI M., GHOLAMI E.. DEVELOPMENT OF RADIOLABELED RECOMBINANT ERYTHROPOIETIN FOR RECEPTOR STUDIES. JOURNAL OF NUCLEAR SCIENCE AND TECHNOLOGY[Internet]. 2012;-(3 (61)):73-79. Available from: https://sid.ir/paper/659030/en

IEEE: Copy

A.R. JALILIAN, H. SADEGHPOUR, M. AKHLAGHI, and E. GHOLAMI, “DEVELOPMENT OF RADIOLABELED RECOMBINANT ERYTHROPOIETIN FOR RECEPTOR STUDIES,” JOURNAL OF NUCLEAR SCIENCE AND TECHNOLOGY, vol. -, no. 3 (61), pp. 73–79, 2012, [Online]. Available: https://sid.ir/paper/659030/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops