BACTERIAL SECRETOME ANALYSIS IN HUNT FOR NOVEL BACTERIOCINS WITH ABILITY TO CONTROL XANTHOMONAS CITRI SUBSP. CITRI

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GHOLAMI DARIUSH; GOODARZI TANNAZ; AMINZADEH SAEED; ALAVI SEYED MEHDI; KAZEMIPOUR NASRIN; FARROKHI NASER

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Journal: IRANIAN JOURNAL OF BIOTECHNOLOGY Year:2015 | Volume:13 | Issue:3 Page(s): 10-19

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Title

BACTERIAL SECRETOME ANALYSIS IN HUNT FOR NOVEL BACTERIOCINS WITH ABILITY TO CONTROL XANTHOMONAS CITRI SUBSP. CITRI

Author(s)

Keywords

BACTERIOCIN

BIOCONTROL

PROTEIN SEQUENCING

TAGUCHI ORTHOGONAL ARRAY

XANTHOMONAS CITRI

Abstract

Background: Xanthomonas citri subsp. citri (Xcc), the causative agent of bacterial citrus canker, has affected citricultureworldwide. Varieties of means have been used to minimize its devastating effects, but no attention has been given tobacteriocins.Objectives: Here and for the first time, we report the isolation and characterization of two novel bacteriocins.Materials and Methods: Secretome containing bacteriocins of isolated bacteria was separated via SDS-PAGE. Each isolatedprotein band was characterized and checked for its efficacy in controlling two pathogenic isolates of Xcc via disk diffusionassay. The effects of varieties of carbon, nitrogen and phosphate sources were evaluated on both bacterial growthand bacteriocin production via Taguchi orthogonal method.Results: The two bacteriocins showed an activity up to 55ºC that were sensitive to proteases suggesting being protein in nature.Analysis of SDS-PAGE purified protein bands of bacterial secretomes with demonstrated potency against Xcc revealed thepresence of peptides with relative molecular masses of 16.9 and 17 kDa for Cronobacter and Enterobacter, respectively.Sequence analysis of peptides revealed an HCP1 family VI secretion system homologue for Cronobacter (YP_001439956) andpilin FimA homologue for Enterobacter (CBK85798.1). A Taguchi orthogonal array was also implemented to determine theeffect of temperature and eight other chemical factors on bacteriocin production for each bacterium.Conclusions: Two peptides with novel antibacterial activities effective against Xcc were isolated, characterized and conditionswere optimized for their higher production.

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APA: Copy

GHOLAMI, DARIUSH, GOODARZI, TANNAZ, AMINZADEH, SAEED, ALAVI, SEYED MEHDI, KAZEMIPOUR, NASRIN, & FARROKHI, NASER. (2015). BACTERIAL SECRETOME ANALYSIS IN HUNT FOR NOVEL BACTERIOCINS WITH ABILITY TO CONTROL XANTHOMONAS CITRI SUBSP. CITRI. IRANIAN JOURNAL OF BIOTECHNOLOGY, 13(3), 10-19. SID. https://sid.ir/paper/684883/en

Vancouver: Copy

GHOLAMI DARIUSH, GOODARZI TANNAZ, AMINZADEH SAEED, ALAVI SEYED MEHDI, KAZEMIPOUR NASRIN, FARROKHI NASER. BACTERIAL SECRETOME ANALYSIS IN HUNT FOR NOVEL BACTERIOCINS WITH ABILITY TO CONTROL XANTHOMONAS CITRI SUBSP. CITRI. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2015;13(3):10-19. Available from: https://sid.ir/paper/684883/en

IEEE: Copy

DARIUSH GHOLAMI, TANNAZ GOODARZI, SAEED AMINZADEH, SEYED MEHDI ALAVI, NASRIN KAZEMIPOUR, and NASER FARROKHI, “BACTERIAL SECRETOME ANALYSIS IN HUNT FOR NOVEL BACTERIOCINS WITH ABILITY TO CONTROL XANTHOMONAS CITRI SUBSP. CITRI,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 13, no. 3, pp. 10–19, 2015, [Online]. Available: https://sid.ir/paper/684883/en

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