BIOCONTROL ACTIVITIES OF GAMMA INDUCED MUTANTS OF TRICHODERMA HARZIANUM AGAINST SOME SOILBORNE FUNGAL PATHOGENS AND THEIR DNA FINGERPRINTING

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ABBASI SAKINEH; SAFAEI NASER; SHAMS BAKHSH MASOUD; SHAHBAZI SAMIRA

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Journal: Iranian Journal of Biotechnology Year:2016 | Volume:14 | Issue:4 Page(s): 260-269

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Title

BIOCONTROL ACTIVITIES OF GAMMA INDUCED MUTANTS OF TRICHODERMA HARZIANUM AGAINST SOME SOILBORNE FUNGAL PATHOGENS AND THEIR DNA FINGERPRINTING

Author(s)

ABBASI SAKINEH | SAFAEI NASER | SHAMS BAKHSH MASOUD | SHAHBAZI SAMIRA | Issue Writer Certificate

Keywords

ENHANCEMENT OF ANTAGONISTIC PROPERTIES

RANDOM MUTAGENESIS

RAPD AND REP-PCR

Abstract

Background: Random induced mutation by gamma radiation is one of the genetic manipulation strategies to improve the antagonistic ability of biocontrol agents.Objectives: This study aimed to induce mutants with more sporulation, colonization rate leading to enhanced antagonistic ability (in vitro assay) comparing to wild type (WT) and the assessment of genetic differences (in situ evaluation) using molecular markers. The superior mutants could be appropriate biocontrol agents against soil borne fungal diseases.Materials and Methods: In this research sampling and isolation of Trichoderma isolates were performed from soils with low incidence of soil borne disease.T. harzianum 65 was selected and irradiation was conducted with gammacell at optimal dose 250 Gray/s. Mutants (115) were obtained from the WT. The antagonistic abilities of twenty-four mutants were evaluated using dual culture and culture filtrate tests.Results: The results of in vitro assays revealed that Th15, Th11 and Th1 mutants exhibited stronger growth inhibition (GI) and colonization rate onMacrophomina phaseolina and Rhizoctonia solani AG4 compared to the wild type. Th15 and Th11 mutants exhibited stronger GI and colonization rate onSclerotinia sclerotiorum in dual culture and culture filtrate tests and Th1and Th11 mutants exhibited stronger GI on Fusarium grminearum in culture filtrate test.The DNA fingerprinting was carried out using RAPD AND REP-PCR markers. Two (Th9 and Th17) out of the 24 mutants categorized distantly from the rest based on different polymorphism obtained by molecular markers. However, Th9 was different in GI% fromTh17. RAPD analysis separated WT from mutants, Th9 from Th17 and also phenotypically superior mutants from other mutants. Meanwhile, rep-PCR analysis categorized WT isolate and mutants according to their antagonistic properties.Conclusions: The latter marker (rep-PCR) appeared to be reproducible and simple to distinguish mutants from a single isolate ofT. harzianum. Mutants (3 isolates) were phenotypically and genotypically distinct from WT. These mutants demonstrated a pronounced biocontrol activities against soilborne fungal phytopathogens.

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APA: Copy

ABBASI, SAKINEH, SAFAEI, NASER, SHAMS BAKHSH, MASOUD, & SHAHBAZI, SAMIRA. (2016). BIOCONTROL ACTIVITIES OF GAMMA INDUCED MUTANTS OF TRICHODERMA HARZIANUM AGAINST SOME SOILBORNE FUNGAL PATHOGENS AND THEIR DNA FINGERPRINTING. IRANIAN JOURNAL OF BIOTECHNOLOGY, 14(4), 260-269. SID. https://sid.ir/paper/684888/en

Vancouver: Copy

ABBASI SAKINEH, SAFAEI NASER, SHAMS BAKHSH MASOUD, SHAHBAZI SAMIRA. BIOCONTROL ACTIVITIES OF GAMMA INDUCED MUTANTS OF TRICHODERMA HARZIANUM AGAINST SOME SOILBORNE FUNGAL PATHOGENS AND THEIR DNA FINGERPRINTING. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2016;14(4):260-269. Available from: https://sid.ir/paper/684888/en

IEEE: Copy

SAKINEH ABBASI, NASER SAFAEI, MASOUD SHAMS BAKHSH, and SAMIRA SHAHBAZI, “BIOCONTROL ACTIVITIES OF GAMMA INDUCED MUTANTS OF TRICHODERMA HARZIANUM AGAINST SOME SOILBORNE FUNGAL PATHOGENS AND THEIR DNA FINGERPRINTING,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 14, no. 4, pp. 260–269, 2016, [Online]. Available: https://sid.ir/paper/684888/en

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