Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

492
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

252
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

1

Information Journal Paper

Title

INDUCTION OF APOPTOSIS IN HUMAN BREAST CANCER MCF-7 CELLS BY A SEMI-SYNTHETIC DERIVATIVE OF ARTEMISININ: A CASPASE-RELATED MECHANISM

Pages

  157-165

Abstract

 Background: ARTESUNATE has recently been used in some pharmacological preparation to induce tumor cell APOPTOSIS. The drug is a semi-synthetic derivative of artemisinin, traditionally used for its antimalarial. However, up to now, its anticancer mechanism against diff erent types of tumors is not known.Objectives: The most important purposes of the present research was fi rstly investigating induction of APOPTOSIS on human BREAST CANCER MCF-7 CELLS by the drug and, in the second place, introducing its possible mechanism of action.Materials and Methods: The MTT assay was used to investigate the inhibitory eff ect of ARTESUNATE on growth of BREAST CANCER MCF-7 CELLS. For this aim, diff erent concentrations of ARTESUNATE were used to treat the cells and fl ow cytometry assay was done followed by annexin V-FITC/PI staining. The activities of caspase-3, -8 and -9 were then determined by relative assay kits.Results: Based on the results from MTT assay, it was found that ARTESUNATE could signifi cantly inhibit the growth of MCF-7 CELLS in a dose- and time-dependent manner. On the other hand, the fl ow cytometry fi ndings showed that the anti-proliferative activity of ARTESUNATE on MCF-7 CELLS is due to APOPTOSIS. Besides, caspase colorimetric assays revealed a signifi cant rise in cellular levels of the initiators (caspase-8 and -9) and eff ector (caspase-3) in the cells treated by ARTESUNATE.Conclusions: According to our results, it could be concluded that ARTESUNATE could inhibit the growth of MCF-7 BREAST CANCER cells through induction of APOPTOSIS by intrinsic and extrinsic caspase-dependent pathways. Therefore, we claim that ARTESUNATE could be introduced as a suitable candidate for the treatment of the BREAST CANCER.

Cites

References

  • No record.

    • Cite

    APA: Copy

    Jamalzadeh, Leila, GHAFOORI, HOSSEIN, AGHAMAALI, MAHMOODREZA, & SARIRI, REYHANEH. (2017). INDUCTION OF APOPTOSIS IN HUMAN BREAST CANCER MCF-7 CELLS BY A SEMI-SYNTHETIC DERIVATIVE OF ARTEMISININ: A CASPASE-RELATED MECHANISM. IRANIAN JOURNAL OF BIOTECHNOLOGY, 15(3), 157-165. SID. https://sid.ir/paper/693028/en

    Vancouver: Copy

    Jamalzadeh Leila, GHAFOORI HOSSEIN, AGHAMAALI MAHMOODREZA, SARIRI REYHANEH. INDUCTION OF APOPTOSIS IN HUMAN BREAST CANCER MCF-7 CELLS BY A SEMI-SYNTHETIC DERIVATIVE OF ARTEMISININ: A CASPASE-RELATED MECHANISM. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2017;15(3):157-165. Available from: https://sid.ir/paper/693028/en

    IEEE: Copy

    Leila Jamalzadeh, HOSSEIN GHAFOORI, MAHMOODREZA AGHAMAALI, and REYHANEH SARIRI, “INDUCTION OF APOPTOSIS IN HUMAN BREAST CANCER MCF-7 CELLS BY A SEMI-SYNTHETIC DERIVATIVE OF ARTEMISININ: A CASPASE-RELATED MECHANISM,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 15, no. 3, pp. 157–165, 2017, [Online]. Available: https://sid.ir/paper/693028/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops





    Move to top