Synthesis of Pirano-Based Piranazole-based Compounds to Induce Apoptosis by Reducing the Expression of Anti-Apoptotic Protein B2 Cell Lymphoma Protein in the MCF-7 Human Breast Cancer Cell Category

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Shahriyari Nejad H.; Pourmohsen N.; GHAFOURI H.; Zareie S.; SHARIFI R.; MAHMOODI N.

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Journal: Armaghane Danesh Year:2020 | Volume:25 | Issue:1(138) Page(s): 40-54

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Title

Synthesis of Pirano-Based Piranazole-based Compounds to Induce Apoptosis by Reducing the Expression of Anti-Apoptotic Protein B2 Cell Lymphoma Protein in the MCF-7 Human Breast Cancer Cell Category

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PyrazoleQ1

ApoptosisQ1

MCF-7 cellsQ1

Bcl-2Q1

Abstract

Background & aim: B-cell lymphoma 2 (Bcl-2) is a potential target for tumor treatment. The inhibition of the Bcl-2 production is research target of attraction in the field of anti-cancer drug development. The present study aimed to evaluate the inhibitory effects of novel Pyrazole derivatives on Bcl-2 expression in human breast cancer cell line MCF-7. Methods: In the present in vitro experimental study, the newly synthesized substances were screened against breast Aden carcinoma (MCF-7). The Western-blot analysis was carried out to study signaling pathways of MCF-7 breast cancer cells. The levels of Apoptosis-related protein (Bcl-2) were evaluated by western blot analysis and changes in it expression were confirmed. Data were analyzed using one-way ANOVA, and independent t-test. Results: The compounds HN1 and HN2 significantly inhibited the proliferation of human breast cancer cells. The compounds HN1and HN2 inhibited the growth of MCF-7 cells with IC50 values of 7. 4 μ g/ml and 8. 68 μ g/ml, respectively. In addition, compounds HN1and HN2 (22. 5-50 μ g/ml) significantly inhibited the anti-apoptotic Bcl-2 protein production. The compound HN2 significantly inhibited Bcl-2 expression in a concentration-dependent manner, corresponding 24% at 37. 5 μ g/ml, 30% at 50 μ g/ml. Also compound HN1 at the same concentrations inhibited Bcl-2 expression by 12%, 0% at 50 and 37/5 μ g/ml in MCF-7 cells, respectively. Conclusion: HN2 could suppress the viability of MCF-7 cells and induce Apoptosis in breast cancer cells by down-regulation of anti-apoptotic factor, Bcl-2. These results revealed that the potential inhibitory effect of HN2 against growth of MCF-7 human breast cancer cells might be associated with induction of Apoptosis through Bcl-2 protein dependent pathway. The present results suggest that HN2 has a promising potential to be used as a valuable chemo preventive agent.

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APA: Copy

Shahriyari Nejad, H., Pourmohsen, N., GHAFOURI, H., Zareie, S., SHARIFI, R., & MAHMOODI, N.. (2020). Synthesis of Pirano-Based Piranazole-based Compounds to Induce Apoptosis by Reducing the Expression of Anti-Apoptotic Protein B2 Cell Lymphoma Protein in the MCF-7 Human Breast Cancer Cell Category. ARMAGHAN DANESH, 25(1(138) ), 40-54. SID. https://sid.ir/paper/397119/en

Vancouver: Copy

Shahriyari Nejad H., Pourmohsen N., GHAFOURI H., Zareie S., SHARIFI R., MAHMOODI N.. Synthesis of Pirano-Based Piranazole-based Compounds to Induce Apoptosis by Reducing the Expression of Anti-Apoptotic Protein B2 Cell Lymphoma Protein in the MCF-7 Human Breast Cancer Cell Category. ARMAGHAN DANESH[Internet]. 2020;25(1(138) ):40-54. Available from: https://sid.ir/paper/397119/en

IEEE: Copy

H. Shahriyari Nejad, N. Pourmohsen, H. GHAFOURI, S. Zareie, R. SHARIFI, and N. MAHMOODI, “Synthesis of Pirano-Based Piranazole-based Compounds to Induce Apoptosis by Reducing the Expression of Anti-Apoptotic Protein B2 Cell Lymphoma Protein in the MCF-7 Human Breast Cancer Cell Category,” ARMAGHAN DANESH, vol. 25, no. 1(138) , pp. 40–54, 2020, [Online]. Available: https://sid.ir/paper/397119/en

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