PRODUCTION OF XANTHANASES BY PAENIBACILLUS SPP.: COMPLETE XANTHAN DEGRADATION AND POSSIBLE APPLICATIONS

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Ashraf Simin; SOUDI MOHAMMAD REZA; GHADAM PARINAZ

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Journal: Iranian Journal of Biotechnology Year:2017 | Volume:15 | Issue:2 Page(s): 0-0

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Title

PRODUCTION OF XANTHANASES BY PAENIBACILLUS SPP.: COMPLETE XANTHAN DEGRADATION AND POSSIBLE APPLICATIONS

Author(s)

Ashraf Simin | SOUDI MOHAMMAD REZA | GHADAM PARINAZ | Issue Writer Certificate

Keywords

BACTERIAL ENZYMES

BIODEGRADATION

PAENIBACILLUS SPP.

XANTHAN LYASE

XANTHANASE

Abstract

Background: A number of microorganisms and their enzymes have been reported as xanthan depolymerizers. Paenibacillus species are well-known polysaccharide hydrolyzing bacteria. However, Paenibacillus alginolyticus and Paenibacillus sp.XD are the only species in the genus which are now known to degrade xanthan.Objectives: Complete BIODEGRADATION of the xanthan exopolysaccharide is a rarely found capability among microorganisms.The aim of this study is to survey XANTHANASE producing bacteria with an appropriate bioactivity for the biopolymer degradation under diff erent environmental conditions.Materials and Methods: The bacteria were isolated based on viscosity reduction of the xanthan solution. Bacterial isolates were identifi ed using rep-PCR (repetitive element-based genomic fingerprinting) and 16S rDNA sequencing. XANTHANASEs were characterized by measuring their activity at different temperatures, pH values, and NaCl concentrations. Degradation of other polysaccharides and xanthan degradation products were investigated based on the screening plate method and TLC (thin-layer chromatography), respectively.Results: Six isolates from different Paenibacillus species with a complete xanthan degrading capability were isolated from Urmia Lake. Phylogenetic analysis placed these strains within the genus Paenibacillus with the closest relatives that were found to beP. nanensis, P. phyllosphaerae, P. agaridevorans, P. agarexedens, and P. taohuashanense. These isolates displayed diff erent levels of the xanthan BIODEGRADATION activity in temperatures ranging from 15 to 55oC and pH values from 4 to 11. Xanthanolytic activity was generally prevented in presence of NaCl (>0.1 mol.L-1). Furthermore, the isolated Paenibacillusspp. could degrade several other polysaccharides including xylan, CMC (carboxymethyl cellulose), starch, alginate, and pectin.Conclusion: Novel strains of the six diff erent Paenibacillus species that were introduced in the present study are able to produce XANTHANASEs with interesting characteristics. In light of the results from this study, special applications, particularly in healthcare, medicine, and the environment is hereby proposed for these enzymes.

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APA: Copy

Ashraf, Simin, SOUDI, MOHAMMAD REZA, & GHADAM, PARINAZ. (2017). PRODUCTION OF XANTHANASES BY PAENIBACILLUS SPP.: COMPLETE XANTHAN DEGRADATION AND POSSIBLE APPLICATIONS. IRANIAN JOURNAL OF BIOTECHNOLOGY, 15(2), 0-0. SID. https://sid.ir/paper/695838/en

Vancouver: Copy

Ashraf Simin, SOUDI MOHAMMAD REZA, GHADAM PARINAZ. PRODUCTION OF XANTHANASES BY PAENIBACILLUS SPP.: COMPLETE XANTHAN DEGRADATION AND POSSIBLE APPLICATIONS. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2017;15(2):0-0. Available from: https://sid.ir/paper/695838/en

IEEE: Copy

Simin Ashraf, MOHAMMAD REZA SOUDI, and PARINAZ GHADAM, “PRODUCTION OF XANTHANASES BY PAENIBACILLUS SPP.: COMPLETE XANTHAN DEGRADATION AND POSSIBLE APPLICATIONS,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 15, no. 2, pp. 0–0, 2017, [Online]. Available: https://sid.ir/paper/695838/en

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