IN VITRO MICROTUBER INDUCTION AND GROWTH OF POTATO (SOLANUM UBEROSUM. L)CV. SANTE, IN RESPONSE TO THE DIFFERENT CONCENTRATIONS BAP AND SUCROSE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

EBADI M.; IRANBAKHSH A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY) Year:2011 | Volume:20 | Issue:78/1 (BIOLOGY ISSU) Page(s): 1-14

Download Full-Text

Persian Verion

View:

979

Download:

Cites:

Information Journal Paper

Title

IN VITRO MICROTUBER INDUCTION AND GROWTH OF POTATO (SOLANUM UBEROSUM. L)CV. SANTE, IN RESPONSE TO THE DIFFERENT CONCENTRATIONS BAP AND SUCROSE

Author(s)

EBADI M. | IRANBAKHSH A. | Issue Writer Certificate

Keywords

POTATOQ3

BAPQ3

SUCROSEQ3

MICROTUBERIZATIONQ3

Abstract

Introduction: POTATO is one of stratigic crops in the world. The production volume this plant in the world has fourth ranked after wheat, rice and corn. Therefore, understanding how to improve its quality of tubers development and controlling factors of tuber initiation and growth is very important.Aims: In the study, the effects of the different concentrations of BAP and SUCROSE, as inducing compounds of MICROTUBERIZATION, was investigated on MICROTUBERIZATION period, number of microtubers, dry weights and fresh weights of microtubers in the tissue culture conditions.Materials and Method: For study of hormonal and suger treatments, two-stage culture was used. In the first stage of MS liquid medium containing 0.5 mgl-1 BAP, 0.4 mgl-1 GA3, and 20 gl-1 SUCROSE was used for shoot formation. The single node explants were growing in white light (5000-4000 LUX) on the Shaker, one month. In the second stage, MS liquid induction MICROTUBERIZATION media with different concentrations of SUCROSE (30, 40, 60, 80 gl-1) and BAP (1, 2, 5, 10 mgl-1) were used in continuous darkness. The MICROTUBERIZATION were investigated within 10 weeks after induction. Results: In the induced media containing low concentrations of SUCROSE (30 gl-1), the BAP concentrations was of no inducing effect on MICROTUBERIZATION. The increased concentration of SUCROSE up to (40 gl-1) and only in high concentration of BAP, the microtubers were induced at the end of 4th week with a short delay. However, the microtubers are developed from the change in apical meristem growth pattern and expansion of sub-apical of stolon. Moreover, the microtubers became larger and began to emerge as attached to rhizome with the increase in the concentration of BAP. The increased SUCROSE concentration was up to 60 gl-1 and even in the low concentrations of BAP, the induction of microtubers occurred within a short delay, during the first two weeks until the sixth week. The microtubers didn't survive in condition room. In high concentrations of SUCROSE and BAP, in addition of the induction of microtubers until the second week, the average numbers of microtubers were influenced. In induction media containing high concentrations of SUCROSE (80 gl-1) and BAP (10 mgl-1), the microtubers dormancy and health were more likely. The most ratio of dry weight of microtubers to dry weight of branches was attained in high concentrations of BAP (10 mgl-1) and SUCROSE (8%). The media containing the high concentrations of BAP (5 mgl-1) and SUCROSE (80 gl-1), were of the most number of microtubers, whereas the maximum fresh weight of microtubers appeared in media containing BAP (5 mgl-1) and SUCROSE (60 gl-1). However, the high concentrations of SUCROSE along with high concentrations of BAP decreased the induction period and decreased the microtubers induction within two weeks. The increased concentrations of SUCROSE and BAP, both, were of significant effects on the changes in microtubers' fresh weights. The high concentrations of SUCROSE were of significant effects on the increase of dry weight.Conclusion: In order to selection of the suitable induction media, not only the number and fresh weight of induced microtubers are to be considered but also other parameters such as health, dormancy period and the proportion of dry weight of microtubers to dry weight of branches should be fully taken into regard.

Cites

No record.

References

No record.

Cite

APA: Copy

EBADI, M., & IRANBAKHSH, A.. (2011). IN VITRO MICROTUBER INDUCTION AND GROWTH OF POTATO (SOLANUM UBEROSUM. L)CV. SANTE, IN RESPONSE TO THE DIFFERENT CONCENTRATIONS BAP AND SUCROSE. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), 20(78/1 (BIOLOGY ISSU)), 1-14. SID. https://sid.ir/paper/70409/en

Vancouver: Copy

EBADI M., IRANBAKHSH A.. IN VITRO MICROTUBER INDUCTION AND GROWTH OF POTATO (SOLANUM UBEROSUM. L)CV. SANTE, IN RESPONSE TO THE DIFFERENT CONCENTRATIONS BAP AND SUCROSE. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY)[Internet]. 2011;20(78/1 (BIOLOGY ISSU)):1-14. Available from: https://sid.ir/paper/70409/en

IEEE: Copy

M. EBADI, and A. IRANBAKHSH, “IN VITRO MICROTUBER INDUCTION AND GROWTH OF POTATO (SOLANUM UBEROSUM. L)CV. SANTE, IN RESPONSE TO THE DIFFERENT CONCENTRATIONS BAP AND SUCROSE,” JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), vol. 20, no. 78/1 (BIOLOGY ISSU), pp. 1–14, 2011, [Online]. Available: https://sid.ir/paper/70409/en

Related Journal Papers