RESEARCH OF CHANGE OF TROPANE ALKALOIDS QUANTITIES IN VEGETATIVE GROWTH, FLOWERING, FULL FLOWERING AND FRUITING STAGES OF: HYOSCYAMUS NIGER AND ASSESSMENT OF SUGER AND ELEMENTS CHANGES ON BIOSYNTHESIS OF THESE ALKALOIDS IN TISSUE CULTURE

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CHALABIAN F.; MAJD A.

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Journal Paper

Paper Information

Journal: JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY) Year:2004 | Volume:13 | Issue:50 Page(s): 4141-4153

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Title

RESEARCH OF CHANGE OF TROPANE ALKALOIDS QUANTITIES IN VEGETATIVE GROWTH, FLOWERING, FULL FLOWERING AND FRUITING STAGES OF: HYOSCYAMUS NIGER AND ASSESSMENT OF SUGER AND ELEMENTS CHANGES ON BIOSYNTHESIS OF THESE ALKALOIDS IN TISSUE CULTURE

Author(s)

CHALABIAN F. | MAJD A. | Issue Writer Certificate

Keywords

HYOSCYAMUSQ4

HYSCYAMUS NIGERQ4

TROPANE ALKALOIDSQ4

TISSUE CULTURE OF HYOSCYAMUS NIGERQ4

Abstract

HYOSCYAMUS niger from Solanaceae family is an important pharmacological plant which maintains TROPANE ALKALOIDS. Presence of 8Tropane alkaloids in this plant is investigated by HPLC apparatus. Different parts of plant including root, stem, leaf, flower and seed in different phases of vegetative growth (tow leaves, five leaves, ten leaves), flowering, full flowering and fruiting have been prepared for this experiment. In root, the highest amount of hyoscyamine and scopolamine production are in vegetative phase, in which ten rosette leaves were formed and root from ontogenical regard has secondary structure. In stem, quantity of scopolamine is more in flowering stage in which the stem is green and flexible and from anatomical point structrure of pith is completed. The highest amount of hyoscyamine is in full flowering phase. In leaf, the highest quantity of scopolamine and hyoscyamine are in full flowering and flowering phases, repectively. In generative phase, flower has the most amount of hyoscyamine and scopolaminy. In fruiting phase, seed has the most quantity of scopolamine and hyoscyamine and immature seeds have more alkaloids than mature and brown seeds. Briefly, in vegetative phase; flower and in fruiting phase, seed have the most quantity of TROPANE ALKALOIDS. In vitro culture of HYOSCYAMUS niger plant was carrided out on B5 medium including 2mg/l NAA and 1mg/l 6-BAP. Explants were supplied from culturing of root, hypocotyl and leaf of sterile seed lings which were growing in B5 medium free hormone. In callus formation and organization, explants of leaf and hypocotyl had better response. Changing the quantity of nitrate, phosphate, calcium and sucrose in B5 medium was made in comparison with base medium, then regenerated roots in different media with various amount of mineral elements and sugar were investigated by HPLC apparatus. Augmentation of nitrate concentration as compared with base medium and the medium with lower nitrate concentration, causes increase of growth rate and differentiation of root, but increase of nitrate nihibits the alkaloid production. Decrease of nitrate concentration is the cause of increase of alkaloid concentration relative to base medium (optimum concentration, 10mm). In medium with more phosphate than base medium, formation and differentiation and growth rate of root is more accelerated than in base medium and medium with lower phosphate concentration. But decrease of phosphate concentration causes an increase in alkaloid production relative to base medium and augmentation of phosphate inhibits alkaloid production (optimum concentration for scopolamine and hyoscyamine, 0.5 mM and 1.1 mM, respectively). About sucrose effect, in must be mentioned that with increase of sucrose concentration, root differentiation accelerates and elongation and diameter of roots increase relative to base medium and medium with lower sucrose concentration. Also, with increase of sucrose, alkaloid biosynthesis augments and with diminution of sucrose. With regard to base medium, alkaloid production will be limited. Augmentation of calcium concentration, causes increase of scopolamine and hyoscyamine biosynthesis (optimum concentration, 2mM), also increase of calcium, increase of growth rate of root. Assessment of alkaloid production rate reveals in all phases HYOSCYAMUS niger produces more scopolamine than hyoscyamine.

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References

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APA: Copy

CHALABIAN, F., & MAJD, A.. (2004). RESEARCH OF CHANGE OF TROPANE ALKALOIDS QUANTITIES IN VEGETATIVE GROWTH, FLOWERING, FULL FLOWERING AND FRUITING STAGES OF: HYOSCYAMUS NIGER AND ASSESSMENT OF SUGER AND ELEMENTS CHANGES ON BIOSYNTHESIS OF THESE ALKALOIDS IN TISSUE CULTURE. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), 13(50), 4141-4153. SID. https://sid.ir/paper/70447/en

Vancouver: Copy

CHALABIAN F., MAJD A.. RESEARCH OF CHANGE OF TROPANE ALKALOIDS QUANTITIES IN VEGETATIVE GROWTH, FLOWERING, FULL FLOWERING AND FRUITING STAGES OF: HYOSCYAMUS NIGER AND ASSESSMENT OF SUGER AND ELEMENTS CHANGES ON BIOSYNTHESIS OF THESE ALKALOIDS IN TISSUE CULTURE. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY)[Internet]. 2004;13(50):4141-4153. Available from: https://sid.ir/paper/70447/en

IEEE: Copy

F. CHALABIAN, and A. MAJD, “RESEARCH OF CHANGE OF TROPANE ALKALOIDS QUANTITIES IN VEGETATIVE GROWTH, FLOWERING, FULL FLOWERING AND FRUITING STAGES OF: HYOSCYAMUS NIGER AND ASSESSMENT OF SUGER AND ELEMENTS CHANGES ON BIOSYNTHESIS OF THESE ALKALOIDS IN TISSUE CULTURE,” JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), vol. 13, no. 50, pp. 4141–4153, 2004, [Online]. Available: https://sid.ir/paper/70447/en

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