CONJUGATION OF MORPHINE VACCINE TO GOLD NANOPARTICLES SYNTHESIZED WITH GLUTAMIC ACID

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ZARE D.; AKBARZADEH A.; TANGESTANINEJAD S.; MOGHADAM M.; BARARPOUR N.

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Journal: JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY) Year:2011 | Volume:21 | Issue:80 (CHEMISTRY ISSUE) Page(s): 15-24

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Title

CONJUGATION OF MORPHINE VACCINE TO GOLD NANOPARTICLES SYNTHESIZED WITH GLUTAMIC ACID

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Keywords

CONJUGATIONQ2

MORPHINE VACCINEQ2

GOLD NANOPARTICLESQ2

GLUTAMIC ACIDQ3

Abstract

Introduction: GOLD NANOPARTICLES are one of the most applicable metal nanoparticles which are widely being used in different fields such as nanomedicine and nanobiotechnology.Aim: The goal of this study is the production of GOLD NANOPARTICLES with appropriate size by the use of GLUTAMIC ACID which is considered as a green method. For diagnostic application the synthesized GOLD NANOPARTICLES would be than conjugated with MORPHINE VACCINE.Materials and Methods: In this experimental study, GOLD NANOPARTICLES were synthesized and coated by chemical reduction method with the use of GLUTAMIC ACID. Formation of GOLD NANOPARTICLES and their size were recognized and measured by UV-Vis spectroscopy, Dynamic light scattering and Transmission electron microscopy. After production and recognition of colloidal GOLD NANOPARTICLES they were changed into suspension by the use of PBS buffer. They were then conjugated with MORPHINE VACCINE and incubated at 4oC for 115 hours. Conjugated nanoparticles were characterization by SDS-PAGE and UV-Vis spectroscopy.Results: the highest absorption of these particles was at 525 nm and their size was about 20 nm formed bands pertaining to conjugated nanoparticles were displaced in compare with standard samples.Conclusion: GLUTAMIC ACID is capable of synthesizing GOLD NANOPARTICLES and since amino acids are intoxic, the nanoparticles which are synthesized by the use of amino acids, would be applicable in medicine and biotechnology. GOLD NANOPARTICLES would have synthesized in this method can be used as adjuvant in MORPHINE VACCINE and other vaccines.

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APA: Copy

ZARE, D., AKBARZADEH, A., TANGESTANINEJAD, S., MOGHADAM, M., & BARARPOUR, N.. (2011). CONJUGATION OF MORPHINE VACCINE TO GOLD NANOPARTICLES SYNTHESIZED WITH GLUTAMIC ACID. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), 21(80 (CHEMISTRY ISSUE)), 15-24. SID. https://sid.ir/paper/70572/en

Vancouver: Copy

ZARE D., AKBARZADEH A., TANGESTANINEJAD S., MOGHADAM M., BARARPOUR N.. CONJUGATION OF MORPHINE VACCINE TO GOLD NANOPARTICLES SYNTHESIZED WITH GLUTAMIC ACID. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY)[Internet]. 2011;21(80 (CHEMISTRY ISSUE)):15-24. Available from: https://sid.ir/paper/70572/en

IEEE: Copy

D. ZARE, A. AKBARZADEH, S. TANGESTANINEJAD, M. MOGHADAM, and N. BARARPOUR, “CONJUGATION OF MORPHINE VACCINE TO GOLD NANOPARTICLES SYNTHESIZED WITH GLUTAMIC ACID,” JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), vol. 21, no. 80 (CHEMISTRY ISSUE), pp. 15–24, 2011, [Online]. Available: https://sid.ir/paper/70572/en

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