ISOLATION OF LIPASE PRODUCING BACTERIA FROM FAT MILK AND OPTIMIZATION OF VARIOUS PARAMETER EFFECT ON LIPASE ENZYME PRODUCTION IN SUBMERGE FERMENTATION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TABATABAEI YAZDI F.; MORTAZAVI S.A.; MORADI S.; VASIEE A.R.; ALIZADEH BEHBAHANI B.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY Year:2017 | Volume:14 | Issue:66 Page(s): 187-202

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,342

Download:

Cites:

Information Journal Paper

Title

ISOLATION OF LIPASE PRODUCING BACTERIA FROM FAT MILK AND OPTIMIZATION OF VARIOUS PARAMETER EFFECT ON LIPASE ENZYME PRODUCTION IN SUBMERGE FERMENTATION

Author(s)

Keywords

FAT MILKQ2

LIPOLITICQ2

BACILLUS SUBTILISQ2

RESPONE SURFASE METHODQ1

Abstract

FAT MILK sample was selected to isolationg LIPOLITIC bacteria.25 strains were isolated from FAT MILK. The best isolate was selected for identifing and further study according to lipase producing with amount of 177 U/ml lipase activities that was the most lipase activity among bacteria strains. This bacterium was identified as a strain of genusBacillus based on morphological and biochemical characterization and 16S rRNA gene sequence. Optimization was done for economical producing of lipase by RESPONE SURFASE METHOD. After optimization, screening of culture parameters was conducted by Placket Burman Design. The effect of oive oil, yeast extract, peptone, Mgcl2, glucose, corn syrop, molase, sesame oil, black seed oil, rotation speed and sunflower oil was investigated for optimization. The optimum lipase activity (574 U/ml) was achieved at optimum levels of factors of sesame oil (25.0w/w), olive oil concentration (22.5 g/L), yeast extract (10 g/l) and Mgcl2 (25 mM/l) (160 rpm, pH 6.5 and 24 h) that lipase activity increased to 3.24-fold.Kinetic parameters, Km and Vmax for the purified lipase was calculated from the Michaelis-Menten plot. The lipase showed lower Km value (1.62 mM) and high Vmax value (0.667 mM/min) than the otherBacillus subtilis.

Cites

No record.

References

No record.

Cite

APA: Copy

TABATABAEI YAZDI, F., MORTAZAVI, S.A., MORADI, S., VASIEE, A.R., & ALIZADEH BEHBAHANI, B.. (2017). ISOLATION OF LIPASE PRODUCING BACTERIA FROM FAT MILK AND OPTIMIZATION OF VARIOUS PARAMETER EFFECT ON LIPASE ENZYME PRODUCTION IN SUBMERGE FERMENTATION. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 14(66), 187-202. SID. https://sid.ir/paper/71657/en

Vancouver: Copy

TABATABAEI YAZDI F., MORTAZAVI S.A., MORADI S., VASIEE A.R., ALIZADEH BEHBAHANI B.. ISOLATION OF LIPASE PRODUCING BACTERIA FROM FAT MILK AND OPTIMIZATION OF VARIOUS PARAMETER EFFECT ON LIPASE ENZYME PRODUCTION IN SUBMERGE FERMENTATION. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY[Internet]. 2017;14(66):187-202. Available from: https://sid.ir/paper/71657/en

IEEE: Copy

F. TABATABAEI YAZDI, S.A. MORTAZAVI, S. MORADI, A.R. VASIEE, and B. ALIZADEH BEHBAHANI, “ISOLATION OF LIPASE PRODUCING BACTERIA FROM FAT MILK AND OPTIMIZATION OF VARIOUS PARAMETER EFFECT ON LIPASE ENZYME PRODUCTION IN SUBMERGE FERMENTATION,” IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, vol. 14, no. 66, pp. 187–202, 2017, [Online]. Available: https://sid.ir/paper/71657/en

Related Journal Papers