مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

207
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

144
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

A Recyclable Poly(ionic liquid)s Enzyme Reactor for Highly Efficient Protein Digestion

Pages

  235-246

Keywords

Poly(ionic liquids) 

Abstract

 One of the most significant tasks for proteomic research and industrial applications, is the preparation of recyclable Enzyme reactor. Herein, a novel recyclable Enzyme reactor has been developed based on monodispersed spherical poly(quaternary ammonium ionic liquid)s particles immobilized trypsin. A new quaternary ammonium Ionic liquids functional monomer was first synthesized. The Ionic liquids functional monomer was then copolymerized with ethylene glycol dimethacrylate by precipitation polymerization. The resultant monodispersed spherical particle showed a large surface area (231 m2/g) and high binding capacity for trypsin (200 mg/g) due to the large surface area and strong interaction. The polymer microsphere loaded trypsin was used as an Enzyme reactor for the digestion of standard protein, semi-complex samples and skim milk, respectively. The results indicated that the Enzyme reactor exhibited highly efficient Protein digestion and excellent stability. The digestion time of the present Ionic liquids Enzyme reactor for the digestion of protein, the solution could be reduced to even 5 min. The Ionic liquids Enzyme reactor showed excellent reusability and could be reused for more than four times. When it was kept at 4 ° C for 12 d, and used for skim milk digestion, the obtained MALDI-TOF score could also reach 88 with 29 matched peptides.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    Xie, Mingxue, Su, Rina, & Deng, Oiliang. (2018). A Recyclable Poly(ionic liquid)s Enzyme Reactor for Highly Efficient Protein Digestion. IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE), 37(3), 235-246. SID. https://sid.ir/paper/717549/en

    Vancouver: Copy

    Xie Mingxue, Su Rina, Deng Oiliang. A Recyclable Poly(ionic liquid)s Enzyme Reactor for Highly Efficient Protein Digestion. IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE)[Internet]. 2018;37(3):235-246. Available from: https://sid.ir/paper/717549/en

    IEEE: Copy

    Mingxue Xie, Rina Su, and Oiliang Deng, “A Recyclable Poly(ionic liquid)s Enzyme Reactor for Highly Efficient Protein Digestion,” IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE), vol. 37, no. 3, pp. 235–246, 2018, [Online]. Available: https://sid.ir/paper/717549/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops





    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button