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Title

GENETIC VARIATION OF STELLATE STURGEON, ACIPENSER STELLATUS, FROM THE NORTH (VOLGA RIVER) AND SOUTH (GORGAN RIVER) OF CASPIAN SEA USING RFLP ANALYSIS OF PCR AMPLIFIED ND 5/6 GENE

Pages

  59-70

Abstract

 Genetic variation of STELLATE STURGEON, ACIPENSER STELLATUS, from the North (Volga river) and South (Gorgan river) CASPIAN SEA was investigated using NADH 5/6 gene and PCR-RFLP analysis. In this study, a total of 60 samples of STELLATE STURGEON from the North and 14 samples from the Southern CASPIAN SEA were collected. MtDNA ND 5/6 gene was amplified by polymerase chain reaction (PCR). PCR-product digested using 31 end nucleases restriction enzyme. Five of the 31 enzymes showed polymorphism (RsaI, HinfI, HpaII, HaeII/, MboI). Totally 18 composite heliotypes among 74 samples were detected. Haplotypes AAAAA, BAAAA, ABABA from the Vo]ga river showed 28.33%, 11.66% frequency, respectively, and from the Gorgan river heliotypes AAAAA, BAAAA showed 64.28% and 14.28% frequency, respectively.Average haplotype and nucleotide diversity within the populations in VOLGA RIVER was 0.88±0.027 and 0.0122 and in Gorgan river was 0.5934±0.144 and 0.00398, respectively. Average nucleotide diversity and nucleotide divergence among populations of North and South CASPIAN SEA was 0.008644 and 0.000542, respectively.Monte-Carlo simulation using 1000 replication showed no significant difference between sampling site in the southern and northern CASPIAN SEA (X2=0.926±0.0083).

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    APA: Copy

    SHAABANI, ALI, POURKAZEMI, MOHAMMAD, REZVANI, S., & VITVITSKAYA, V.. (2003). GENETIC VARIATION OF STELLATE STURGEON, ACIPENSER STELLATUS, FROM THE NORTH (VOLGA RIVER) AND SOUTH (GORGAN RIVER) OF CASPIAN SEA USING RFLP ANALYSIS OF PCR AMPLIFIED ND 5/6 GENE. JOURNAL OF MARINE SCIENCES AND TECHNOLOGY, 2(4), 59-70. SID. https://sid.ir/paper/74546/en

    Vancouver: Copy

    SHAABANI ALI, POURKAZEMI MOHAMMAD, REZVANI S., VITVITSKAYA V.. GENETIC VARIATION OF STELLATE STURGEON, ACIPENSER STELLATUS, FROM THE NORTH (VOLGA RIVER) AND SOUTH (GORGAN RIVER) OF CASPIAN SEA USING RFLP ANALYSIS OF PCR AMPLIFIED ND 5/6 GENE. JOURNAL OF MARINE SCIENCES AND TECHNOLOGY[Internet]. 2003;2(4):59-70. Available from: https://sid.ir/paper/74546/en

    IEEE: Copy

    ALI SHAABANI, MOHAMMAD POURKAZEMI, S. REZVANI, and V. VITVITSKAYA, “GENETIC VARIATION OF STELLATE STURGEON, ACIPENSER STELLATUS, FROM THE NORTH (VOLGA RIVER) AND SOUTH (GORGAN RIVER) OF CASPIAN SEA USING RFLP ANALYSIS OF PCR AMPLIFIED ND 5/6 GENE,” JOURNAL OF MARINE SCIENCES AND TECHNOLOGY, vol. 2, no. 4, pp. 59–70, 2003, [Online]. Available: https://sid.ir/paper/74546/en

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