An Electrochemical Genosensor for a Fragment of Apolipoprotein E gene based on Reduced Graphene and Cerium Oxide Nanocomposite

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Jafari Safiye; FARIDBOD FARNOUSH; Haji Hashemi Hedieh

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Journal Paper

Paper Information

Journal: ANALYTICAL AND BIOANALYTICAL ELECTROCHEMISTRY Year:2019 | Volume:11 | Issue:11 Page(s): 1625-1637

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Title

An Electrochemical Genosensor for a Fragment of Apolipoprotein E gene based on Reduced Graphene and Cerium Oxide Nanocomposite

Author(s)

Jafari Safiye | FARIDBOD FARNOUSH | Haji Hashemi Hedieh | Issue Writer Certificate

Keywords

DNA biosensor

Genosensor

Apolipoprotein E gene

Cerium oxide nanoparticles

Reduced graphene oxide

Square wave voltammetry

Abstract

A genosensor was fabricated for detecting a fragment of Apolipoprotein E gene (ApoE). The sensor was made of a nanocomposite of reduced graphene oxide (rGO) and cerium oxide (CeO2) nanoparticles. For this purpose, surface of a glassy carbon electrode was first modified by polyaniline (PANI) and then rGO-CeO2 nanocomposite. It was next characterized through scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. A 23-base oligonucleotide sequences previously reported as a fragment of Apolipoprotein E gene (ApoE) which has a point mutation occurred in the progression of Alzheimer’ s disease was selected as a target molecule. Its ssDNA probe was immobilized on the modified surface via a strong interactions between CeO2 nanoparticles and the phosphate groups in the ssDNA. Detection of the biosensor signal was performed by square wave voltammetry (SWV) and Ru(bpy)3]2+/3+ as an electrochemical probe. In presence and absence of the target sequence, the electrochemical probe showed a different behaviour on the electrode surface. The developed genosensor could detect the ApoE gene sequence high selectively and sensitively, with in a good linear range of 10 fM to 10 nM.

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APA: Copy

Jafari, Safiye, FARIDBOD, FARNOUSH, & Haji Hashemi, Hedieh. (2019). An Electrochemical Genosensor for a Fragment of Apolipoprotein E gene based on Reduced Graphene and Cerium Oxide Nanocomposite. ANALYTICAL & BIOANALYTICAL ELECTROCHEMISTRY, 11(11), 1625-1637. SID. https://sid.ir/paper/773423/en

Vancouver: Copy

Jafari Safiye, FARIDBOD FARNOUSH, Haji Hashemi Hedieh. An Electrochemical Genosensor for a Fragment of Apolipoprotein E gene based on Reduced Graphene and Cerium Oxide Nanocomposite. ANALYTICAL & BIOANALYTICAL ELECTROCHEMISTRY[Internet]. 2019;11(11):1625-1637. Available from: https://sid.ir/paper/773423/en

IEEE: Copy

Safiye Jafari, FARNOUSH FARIDBOD, and Hedieh Haji Hashemi, “An Electrochemical Genosensor for a Fragment of Apolipoprotein E gene based on Reduced Graphene and Cerium Oxide Nanocomposite,” ANALYTICAL & BIOANALYTICAL ELECTROCHEMISTRY, vol. 11, no. 11, pp. 1625–1637, 2019, [Online]. Available: https://sid.ir/paper/773423/en

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