A THERMODYNAMIC STUDY OF THE INTERACTION BETWEEN UREASE AND COPPER IONS

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SABOURI A.A.; POURAKBAR ESFAHANI E.; REZAEI BEHBAHANI G.

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Journal: Journal of Sciences, Islamic Republic of Iran Year:2010 | Volume:21 | Issue:1 Page(s): 15-20

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Title

A THERMODYNAMIC STUDY OF THE INTERACTION BETWEEN UREASE AND COPPER IONS

Author(s)

SABOURI A.A. | POURAKBAR ESFAHANI E. | REZAEI BEHBAHANI G. | Issue Writer Certificate

Keywords

UREASEQ3

COPPER IONQ3

ISOTHERMAL TITRATION CALORIMETRYQ3

ENTHALPY OF BINDINGQ3

ENTROPY OF BINDINGQ3

Abstract

A thermodynamic study of COPPER IONs by jack bean UREASE (JBU) was carried out at two temperatures of 27 and 37°C in Tris buffer (30 mM; pH=7.0) using an ISOTHERMAL TITRATION CALORIMETRY. There is a set of twelve identical and noninteracting binding sites for COPPER IONs. The intrinsic dissociation equilibrium constant and the molar ENTHALPY OF BINDING are 285 mM and -15.2 kJ/mol at 27°C and 346 mM and -14.6 kJ/mol at 37°C, respectively. The molar ENTROPY OF BINDING is 17.2 J K-1 mol-1 at 27°C and +19.1 J K-1 mol-1 at 37°C. Hence, the binding process of COPPER ION to JBU is not only enthalpy driven but also it is entropy driven, which the role of entropy driven should be more effective by increasing the temperature.

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APA: Copy

SABOURI, A.A., POURAKBAR ESFAHANI, E., & REZAEI BEHBAHANI, G.. (2010). A THERMODYNAMIC STUDY OF THE INTERACTION BETWEEN UREASE AND COPPER IONS. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, 21(1), 15-20. SID. https://sid.ir/paper/84078/en

Vancouver: Copy

SABOURI A.A., POURAKBAR ESFAHANI E., REZAEI BEHBAHANI G.. A THERMODYNAMIC STUDY OF THE INTERACTION BETWEEN UREASE AND COPPER IONS. JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN[Internet]. 2010;21(1):15-20. Available from: https://sid.ir/paper/84078/en

IEEE: Copy

A.A. SABOURI, E. POURAKBAR ESFAHANI, and G. REZAEI BEHBAHANI, “A THERMODYNAMIC STUDY OF THE INTERACTION BETWEEN UREASE AND COPPER IONS,” JOURNAL OF SCIENCES ISLAMIC REPUBLIC OF IRAN, vol. 21, no. 1, pp. 15–20, 2010, [Online]. Available: https://sid.ir/paper/84078/en

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