ASSESSMENT OF THE IGE-MEDIATED IMMUNE RESPONSE TO MILK-SPECIFIC NATIVE AND RECOMBINANT PROTEIN IN ALLERGIC PATIENTS BY USING MICROARRAYS

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GAUDIN J.CH.; RABESONA H.; CHOISET H.; CHOBERT J.M.; DROUET M.; HAERTLE T.

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Seminar: BIENNIAL CONGRESS ON IMMUNOLOGY, ASTHMA AND ALLERGY
Year:2007 | Issue:6

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Title

ASSESSMENT OF THE IGE-MEDIATED IMMUNE RESPONSE TO MILK-SPECIFIC NATIVE AND RECOMBINANT PROTEIN IN ALLERGIC PATIENTS BY USING MICROARRAYS

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Abstract

Cow’s milk allergy (CMA) is one of the most widespread human allergies, especially in very young children. Though CMA is the most studied, little is known about the recognition of milk allergen patterns in allergic patients as a prerequisite for the development of efficient diagnostic and prognostic tools. Several factors explain the difficulties of such studies, since (i) milk contains a large number of potential allergens; (ii) the majority of these allergens consist of complex suspensions rather than solutions; (iii) the major allergens such as caseins can’t be purified to homogeneity in large amounts. To override these difficulties we developed a sensitive microarray assay that in combination with near-infrared fluorescence detection was used to study the immune response to milk and purified native and recombinant proteins. This new assay allowed us to assess the binding ability of a large number of allergens to the serum IgE pool of young patients using lower amount of the clinical material. Our data showed that bovine lactoferrin belongs to strong milk allergens. We confirmed that bovine caseins are the main allergens in milk and a-S1 was more representative than a-S2, b and k, which were recognized with almost similar frequency by sera of patients. The recombinant a-S1, a-S2, b and k-caseins caseins purified from the /Escherichia coli/ host, which does not provide phosphorylation and glycosylation of these proteins, were recognized with the same efficiency as their native counterparts by patients’ IgE. Recombinant caseins devoid of post-translational modifications, appear to be useful for the development of /in vitro/ and /in vivo/ diagnostic tools of CMA, especially in microarray-based diagnostic tools.

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APA: Copy

GAUDIN, J.CH., RABESONA, H., CHOISET, H., CHOBERT, J.M., DROUET, M., & HAERTLE, T.. (2007). ASSESSMENT OF THE IGE-MEDIATED IMMUNE RESPONSE TO MILK-SPECIFIC NATIVE AND RECOMBINANT PROTEIN IN ALLERGIC PATIENTS BY USING MICROARRAYS. BIENNIAL CONGRESS ON IMMUNOLOGY, ASTHMA AND ALLERGY. SID. https://sid.ir/paper/902555/en

Vancouver: Copy

GAUDIN J.CH., RABESONA H., CHOISET H., CHOBERT J.M., DROUET M., HAERTLE T.. ASSESSMENT OF THE IGE-MEDIATED IMMUNE RESPONSE TO MILK-SPECIFIC NATIVE AND RECOMBINANT PROTEIN IN ALLERGIC PATIENTS BY USING MICROARRAYS. 2007. Available from: https://sid.ir/paper/902555/en

IEEE: Copy

J.CH. GAUDIN, H. RABESONA, H. CHOISET, J.M. CHOBERT, M. DROUET, and T. HAERTLE, “ASSESSMENT OF THE IGE-MEDIATED IMMUNE RESPONSE TO MILK-SPECIFIC NATIVE AND RECOMBINANT PROTEIN IN ALLERGIC PATIENTS BY USING MICROARRAYS,” presented at the BIENNIAL CONGRESS ON IMMUNOLOGY, ASTHMA AND ALLERGY. 2007, [Online]. Available: https://sid.ir/paper/902555/en

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