COMPARISON OF RAST AND UNICAP 100 IN DETERMINING OF COW'S MILK PROTEIN SPECIFIC IGE

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SHOKOUHI SHOUR MASTI R.; POURPAK Z.; AKRAMIAN R.; MANSOURI MAHBOUBEH; KAMALI SAMINEH

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Seminar Paper

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Seminar: BIENNIAL CONGRESS ON IMMUNOLOGY, ASTHMA AND ALLERGY
Year:2007 | Issue:6

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Title

COMPARISON OF RAST AND UNICAP 100 IN DETERMINING OF COW'S MILK PROTEIN SPECIFIC IGE

Author(s)

SHOKOUHI SHOUR MASTI R. | POURPAK Z. | AKRAMIAN R. | MANSOURI MAHBOUBEH | KAMALI SAMINEH

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Abstract

Background: UniCAP is an automatic autoanalyser that can be used for determining total and specific IgE in allergic diseases, and RAST (Radioallergosorbent test) is a classical technique for specific IgE.Objective: The objective of this study was to compare the results obtained from RAST and CAP system in serum patients with Cow's milk allergy.Materials and Methods: The population of this study consisted of 24 patients with cow's milk allergy. All the patients had clear history of anaphylaxis to cow's milk protein. A blood sample was taken from each patient and both RAST (Allergopharma) and UniCAP 100 (Phadia) were performed for determining specific IgE to cow's milk protein (on the basis of KU/L and Grade 0-5). Finally, the results were compared with t test.Results: Our patients were 15 males and 9 females. The values for Cow's milk specific IgE determined by RAST ranged from 0 to 71.00 KU/L (mean =5.85KU/L, Grade= 0-4) and by UniCAP 100 were from 0.25 to 95.40 (mean=18.69KU/L, Grade=0-5). A significant difference (P=0.005) was between mean of specific IgE in methods of RAST and UniCAP 100.Conclusion: It seems that UniCAP is more precise than RAST in determining specific IgE in serum of patients with cow's milk allergy. It can owe solid phase of UniCAP system.

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APA: Copy

SHOKOUHI SHOUR MASTI, R., POURPAK, Z., AKRAMIAN, R., MANSOURI, MAHBOUBEH, & KAMALI, SAMINEH. (2007). COMPARISON OF RAST AND UNICAP 100 IN DETERMINING OF COW'S MILK PROTEIN SPECIFIC IGE. BIENNIAL CONGRESS ON IMMUNOLOGY, ASTHMA AND ALLERGY. SID. https://sid.ir/paper/902588/en

Vancouver: Copy

SHOKOUHI SHOUR MASTI R., POURPAK Z., AKRAMIAN R., MANSOURI MAHBOUBEH, KAMALI SAMINEH. COMPARISON OF RAST AND UNICAP 100 IN DETERMINING OF COW'S MILK PROTEIN SPECIFIC IGE. 2007. Available from: https://sid.ir/paper/902588/en

IEEE: Copy

R. SHOKOUHI SHOUR MASTI, Z. POURPAK, R. AKRAMIAN, MAHBOUBEH MANSOURI, and SAMINEH KAMALI, “COMPARISON OF RAST AND UNICAP 100 IN DETERMINING OF COW'S MILK PROTEIN SPECIFIC IGE,” presented at the BIENNIAL CONGRESS ON IMMUNOLOGY, ASTHMA AND ALLERGY. 2007, [Online]. Available: https://sid.ir/paper/902588/en

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