EVIDENCE FOR INCORPORATION OF HEPATOCYTE ROUGH ENDOPLASMIC RETICULUM CHLORIDE CHANNEL IN BILAYER LIPID MEMBRANE BASED ON SINGLE CHANNEL RECORDING

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ASHRAFPOUR M.; ELIASI AFSANEH; SAGHIRI R.; FAHANIK BABAEI J.; SEPEHRI HAMID

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Seminar Paper

Paper Information

Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Year:2009 | Issue:19

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Title

EVIDENCE FOR INCORPORATION OF HEPATOCYTE ROUGH ENDOPLASMIC RETICULUM CHLORIDE CHANNEL IN BILAYER LIPID MEMBRANE BASED ON SINGLE CHANNEL RECORDING

Author(s)

ASHRAFPOUR M. | ELIASI AFSANEH | SAGHIRI R. | FAHANIK BABAEI J. | SEPEHRI HAMID

Keywords

SINGLE CHANNEL RECORDING

CHLORIDE CHANNEL

BILAYER LIPID MEMBRANE

HEPATOCYTE

ENDOPLASMIC RETICULUM

Abstract

Many types of Cl- channels have been reported in cell membranes and various types of intracellular organelles including endoplasmic reticulum (ER). Although the exact role of these channels is not clear, it has been suggested that it might contribute to counter currents during Ca2+ release and uptake and regulate transmembrane ion fluxes. Studies designed to determine functional characteristics of channels can help understand their performance and expand perception of cell physiology. The ER was extracted from rat liver by homogenization and ultra centrifugation for several times. Bilayer lipid membrane (BLM) was formed using phosphatidyl choline on 250 μm diameter aperture in between Cis and Trans chambers containing KCL 200 and 50 mM respectively. ER vesicles were incorporated into BLM and then Cl- channel activity was studied using single channel recording technique. The studied Cl- channel current amplitude was equivalent to 6 pA. The conductance was about 105 picosiemens and thecurrentvoltage relationship curve was also linear. The channel had a voltage-independent behavior and its open probability (Po) was low being around 0.07. Channel activity was inhibited by DIDS with a concentration of 0.1 mM. Because the channel open probability was low in different voltages and was not affected by voltage changes, the studied channel is probably a non-voltage-dependent channel and gating kinetic behavior is controlled by ligands.

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APA: Copy

ASHRAFPOUR, M., ELIASI, AFSANEH, SAGHIRI, R., FAHANIK BABAEI, J., & SEPEHRI, HAMID. (2009). EVIDENCE FOR INCORPORATION OF HEPATOCYTE ROUGH ENDOPLASMIC RETICULUM CHLORIDE CHANNEL IN BILAYER LIPID MEMBRANE BASED ON SINGLE CHANNEL RECORDING. IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY. SID. https://sid.ir/paper/903645/en

Vancouver: Copy

ASHRAFPOUR M., ELIASI AFSANEH, SAGHIRI R., FAHANIK BABAEI J., SEPEHRI HAMID. EVIDENCE FOR INCORPORATION OF HEPATOCYTE ROUGH ENDOPLASMIC RETICULUM CHLORIDE CHANNEL IN BILAYER LIPID MEMBRANE BASED ON SINGLE CHANNEL RECORDING. 2009. Available from: https://sid.ir/paper/903645/en

IEEE: Copy

M. ASHRAFPOUR, AFSANEH ELIASI, R. SAGHIRI, J. FAHANIK BABAEI, and HAMID SEPEHRI, “EVIDENCE FOR INCORPORATION OF HEPATOCYTE ROUGH ENDOPLASMIC RETICULUM CHLORIDE CHANNEL IN BILAYER LIPID MEMBRANE BASED ON SINGLE CHANNEL RECORDING,” presented at the IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY. 2009, [Online]. Available: https://sid.ir/paper/903645/en

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