ANTIOXIDANT ENZYMES GENE EXPRESSION DURING AGING IN RAT BRAIN

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ZHALEH HOSSEIN; YOUSEFVAND NADER; YARANI REZA; RASHIDI Z.

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Seminar Paper

Paper Information

Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Year:2009 | Issue:19

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Title

ANTIOXIDANT ENZYMES GENE EXPRESSION DURING AGING IN RAT BRAIN

Author(s)

ZHALEH HOSSEIN | YOUSEFVAND NADER | YARANI REZA | RASHIDI Z.

Keywords

ANTIOXIDANT ENZYMES

AGING

BRAIN

RAT

Abstract

It has been accepted that oxidative damage plays an important role in the AGING process but the effect of age on the gene expression of ANTIOXIDANT ENZYMES has not been extensively studied and findings are contradictory. In this study RATs were divided into two groups, Young group (9 months, n = 7) and old group (24 months, n = 7). Animals were sacrificed under deep anesthesia (Ketamine, 100 mg/kg, I.P). BRAIN of animals were dissected out and cleaned of connective tissues. BRAIN tissues were immersed inRNALater (Ambion, Austin, TX) overnight at 4°C before storage at –20°C until analysis. RNA extraction was performed using the RNeasy Plus Mini Kit (QIAGEN) following the manufacturer’s protocol and after cDNA synthesis. We compared the levels of expression three genes encoding the ANTIOXIDANT ENZYMES in young and old RAT BRAIN, using semiquantitative RT-PCR and analyzing results on a 1% agarose gel. Results showed AGING of RATs led to an increase of catalase Mrna expression. There was a slight, but significant 20% decrease in MnSOD mRNA during AGING. An increase of 40% in Gpx mRNA expression generally occurred during RATs AGING. This experiment supported a significant difference in gene expression in old RAT BRAIN compared to young RAT for catalase and superoxide dismutase, and glutathione peroxidase genes.

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APA: Copy

ZHALEH, HOSSEIN, YOUSEFVAND, NADER, YARANI, REZA, & RASHIDI, Z.. (2009). ANTIOXIDANT ENZYMES GENE EXPRESSION DURING AGING IN RAT BRAIN. IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY. SID. https://sid.ir/paper/903701/en

Vancouver: Copy

ZHALEH HOSSEIN, YOUSEFVAND NADER, YARANI REZA, RASHIDI Z.. ANTIOXIDANT ENZYMES GENE EXPRESSION DURING AGING IN RAT BRAIN. 2009. Available from: https://sid.ir/paper/903701/en

IEEE: Copy

HOSSEIN ZHALEH, NADER YOUSEFVAND, REZA YARANI, and Z. RASHIDI, “ANTIOXIDANT ENZYMES GENE EXPRESSION DURING AGING IN RAT BRAIN,” presented at the IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY. 2009, [Online]. Available: https://sid.ir/paper/903701/en

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