COMPARATIVE EFFECT OF DIFFERENT FACTORS ON MAINTENANCE OF HORSE EMBRYONIC STEM CELLS

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ABAVISANI A.; BAGHBANZADEH A.; GUO J.

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Seminar Paper

Paper Information

Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Year:2009 | Issue:19

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Title

COMPARATIVE EFFECT OF DIFFERENT FACTORS ON MAINTENANCE OF HORSE EMBRYONIC STEM CELLS

Author(s)

ABAVISANI A. | BAGHBANZADEH A. | GUO J.

Keywords

EQUINE EMBRYONIC STEM CELLS

HUMAN LEUKEMIA INHIBITORY FACTOR

HUMAN BASIC FIBROBLASTIC GROWTH FACTOR

MAINTENANCE

Abstract

Embryonic stem cells (ESCs) are originally derived from the ICM of blastocysts and characterized by their ability to self-renew and their pluripotencies. Although attempt to isolate equine ESCs has been done, proper growth factor(s) for their MAINTENANCE have not been still established. Different growth factors or even the same factors play different roles in ESC culture in different species. Our aim was to determine the influence of hLIF and bFGF on the horse embryonic stem cell culture. The putative eESC (equine ESCs) cell lines were derived from day- 7 blastocyst ICMs, mechanically. The growth capabilities of the eESCs were evaluated when these cells were cultured in the basic medium supplemented with HUMAN LEUKEMIA INHIBITORY FACTOR (hLIF) or HUMAN BASIC FIBROBLASTIC GROWTH FACTOR (hbFGF) on mouse embryonic firoblasts. The culture medium was changed every day and the passage was performed by mechanical method every 6-8 days. Up to passage 15, cell morphology of putative eESCs could be maintained in the basic medium supplemented with hLIF, whereas medium supplemented with bFGF could not support them. Immunostaning of these colonies showed that some cell s within the colonies were positive for Oct3/4, SSEA-1, TRA 1-60, TRA1-80 and GCTM-2. So, these results suggest that hLIF supports the MAINTENANCE of eESC stemness but bFGF is negative for MAINTENANCE of their pluripotecy.

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APA: Copy

ABAVISANI, A., BAGHBANZADEH, A., & GUO, J.. (2009). COMPARATIVE EFFECT OF DIFFERENT FACTORS ON MAINTENANCE OF HORSE EMBRYONIC STEM CELLS. IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY. SID. https://sid.ir/paper/903723/en

Vancouver: Copy

ABAVISANI A., BAGHBANZADEH A., GUO J.. COMPARATIVE EFFECT OF DIFFERENT FACTORS ON MAINTENANCE OF HORSE EMBRYONIC STEM CELLS. 2009. Available from: https://sid.ir/paper/903723/en

IEEE: Copy

A. ABAVISANI, A. BAGHBANZADEH, and J. GUO, “COMPARATIVE EFFECT OF DIFFERENT FACTORS ON MAINTENANCE OF HORSE EMBRYONIC STEM CELLS,” presented at the IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY. 2009, [Online]. Available: https://sid.ir/paper/903723/en

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