MG2+ PROTECTS ADULT BEATING CARDIOMYOCYTES AGAINST ISCHAEMIA

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FAGHIHI M.; SUKHODUB A.; JOVANOVIC S.; JOVANOVIC A.

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Seminar Paper

Paper Information

Seminar: IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY
Year:2007 | Issue:18

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Title

MG2+ PROTECTS ADULT BEATING CARDIOMYOCYTES AGAINST ISCHAEMIA

Author(s)

FAGHIHI M. | SUKHODUB A. | JOVANOVIC S. | JOVANOVIC A.

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Abstract

Although Mg2+ reduces infarct size in whole heart models of ischaemia/reperfusion, the cardioprotective effect of Mg2+ at the cellular level is still a controversial issue. Therefore, we have tested whether Mg2+ protects cardiomyocytes against ischaemia. To address this aim we have used single cell model of ischaemia that utilises single beating adult cardiomyocytes in which oxygen tension is tightly regulated without use of oxygen scavengers or metabolic inhibitors, which, all together, makes this model closer to in vivo conditions than the majority of previously published cellular models of ischaemia. We have found that extracellular addition of the Mg2+ significantly increased survival of cells exposed to ischaemia (under control conditions cells survived 16.8±5.8 min and 66.5±11.0 min when 8mM of MgSO4 was added). However, Mg2+ (8 mM) did not affect whole cell K+ current as revealed by patch clamp electrophysiology. Experiments with laser confocal microscopy and mitochondrial membrane potential-sensitive dye, JC-1, have shown that Mg2+ (8mM) did not affect ischaemia-induced mitochondrial membrane depolarisation (in the absence of Mg2+ JC-1 ratio have rose from 0.21±0.03 to 0.48±0.11 and from 0.22±0.08 to 0.60±0.21 in the presence of Mg2+, P>0.05 when compared JC-1 ratios with and without Mg2+). However, a significantly lower JC-1 ratio was required to kill cells under control conditions than those treated with 8mM MgSO4 (0.48±0.11 vs 0.96±0.21). It is concluded that Mg2+ protect single beating cardiomyocytes against ischaemia by increasing cellular resistance to the consequences of mitochondrial membrane depolarisation.

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APA: Copy

FAGHIHI, M., SUKHODUB, A., JOVANOVIC, S., & JOVANOVIC, A.. (2007). MG²⁺ PROTECTS ADULT BEATING CARDIOMYOCYTES AGAINST ISCHAEMIA. IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY. SID. https://sid.ir/paper/904723/en

Vancouver: Copy

FAGHIHI M., SUKHODUB A., JOVANOVIC S., JOVANOVIC A.. MG²⁺ PROTECTS ADULT BEATING CARDIOMYOCYTES AGAINST ISCHAEMIA. 2007. Available from: https://sid.ir/paper/904723/en

IEEE: Copy

M. FAGHIHI, A. SUKHODUB, S. JOVANOVIC, and A. JOVANOVIC, “MG²⁺ PROTECTS ADULT BEATING CARDIOMYOCYTES AGAINST ISCHAEMIA,” presented at the IRANIAN CONGRESS OF PHYSIOLOGY AND PHARMACOLOGY. 2007, [Online]. Available: https://sid.ir/paper/904723/en

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