ASSESSMENT OF BIODIVERSITY (POLYMORPHISM) AMONG XANTHOMONAS SPP. BY ERIC PCR

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GHASHGHAEI TAHEREH; ASGARANI EZAT; SOUDI MOHAMMAD REZA; SALMANZADEH AHRABI SIAVASH

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Seminar Paper

Paper Information

Seminar: CONGRESS OF IRANIAN GENETICS
Year:2014 | Issue:1

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Title

ASSESSMENT OF BIODIVERSITY (POLYMORPHISM) AMONG XANTHOMONAS SPP. BY ERIC PCR

Author(s)

GHASHGHAEI TAHEREH | ASGARANI EZAT | SOUDI MOHAMMAD REZA | SALMANZADEH AHRABI SIAVASH

Keywords

BIODIVERSITY

ERIC PCR

XANTHOMONAS SPECIES

Abstract

THE GENUS OF THE XANTHOMONAS IS COMPOSED OF PHYTOPATHOGENIC BACTERIAL SPECIES THAT CAUSE DISEASE IN DIFFERENT CROP PLANTS, RESULTING IN SIGNIFICANT CROP LOSSES WORLDWIDE. EACH SPECIES INFECTS SPECIFIC HOSTS. IN ADDITION TO CAUSING DISEASE IN CROPS, MOST OF THE SPECIES PRODUCE XANTHAN GUM VIA AN AEROBIC FERMENTATION PROCESS. XANTHAN GUM IS AN IMPORTANT BIOPOLYMER AND IS USED IN THE FOOD, PETROLEUM, AND COSMETIC INDUSTRIES. MANY STUDIES HAVE USED MOLECULAR BIOLOGY TECHNIQUES WITH DIVERSE XANTHOMONAS SPECIES TO SHOW HIGH LEVELS OF GENETIC DIVERSITY (POLYMORPHISM) IN THE GENUS AS WELL AS WITHIN SPECIES. ON THE OTHER HAND, PATHOVARS FROM DIFFERENT SPECIES HAVE SHOWN STRONG SIMILARITIES, RESULTING IN A TOTAL RECLASSIFICATION OF THE GENUS. IN THIS STUDY BIODIVERSITY OF XANTHOMONAS SPP. WAS ASSESSED BY ERIC PCR. IN FACT FAMILIES OF SHORT INTERSPERSED REPETITIVE ELEMENTS HAVE BEEN DESCRIBED IN BACTERIA, I.E., ENTEROBACTERIAL REPETITIVE INTERGENIC CONSENSUS (ERIC) SEQUENCE. SEQUENCE DATA FOR ERIC ELEMENT HAVE BEEN DESCRIBED ONLY FROM GRAM NEGATIVE ENTERIC BACTERIA AND CLOSELY RELATED TAXA. IN THIS STUDY, WE ASSESSED PROFILE OF 15 XANTHOMONAS STRAINS. FIRST TOTAL BACTERIAL DNA EXTRACTED BY PHENOL-CHLOROFORM METHOD. PRIMERS TO ERIC SEQUENCES HAVE BEEN USED IN POLYMERASE CHAIN REACTIONS DESIGNED TO AMPLIFY REGIONS BETWEEN NEIGHBORING REPETITIVE ELEMENTS. OUR RESULTS SHOWED BACTERIA HAVE DIFFERENT PATTERN OF BANDS ON THE GEL AFTER ELECTROPHORESIS. SOME BANDS ON THE ELECTROPHORESIS GEL WERE THE SAME AMONG THESE STRAINS AND OTHER BANDS WERE DIFFERENT. THIS METHOD SHOWED UNIQUE PROFILE FOR EACH STRAIN. THAT MEANS THESE BACTERIA HAVE DIFFERENT GENOTYPES AND EXHIBIT SOME LEVELS OF POLYMORPHISMS. IN FACT ERIC- PCR IS USEFUL AND RAPID METHOD FOR DIFFERENTIATION AMONG XANTHOMONAS SPECIES.

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APA: Copy

GHASHGHAEI, TAHEREH, ASGARANI, EZAT, SOUDI, MOHAMMAD REZA, & SALMANZADEH AHRABI, SIAVASH. (2014). ASSESSMENT OF BIODIVERSITY (POLYMORPHISM) AMONG XANTHOMONAS SPP. BY ERIC PCR. CONGRESS OF IRANIAN GENETICS. SID. https://sid.ir/paper/906876/en

Vancouver: Copy

GHASHGHAEI TAHEREH, ASGARANI EZAT, SOUDI MOHAMMAD REZA, SALMANZADEH AHRABI SIAVASH. ASSESSMENT OF BIODIVERSITY (POLYMORPHISM) AMONG XANTHOMONAS SPP. BY ERIC PCR. 2014. Available from: https://sid.ir/paper/906876/en

IEEE: Copy

TAHEREH GHASHGHAEI, EZAT ASGARANI, MOHAMMAD REZA SOUDI, and SIAVASH SALMANZADEH AHRABI, “ASSESSMENT OF BIODIVERSITY (POLYMORPHISM) AMONG XANTHOMONAS SPP. BY ERIC PCR,” presented at the CONGRESS OF IRANIAN GENETICS. 2014, [Online]. Available: https://sid.ir/paper/906876/en

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