SCREENING OF CYCLOTIDES AS ANTIMICROBIAL PEPTIDES FROM SEVERAL VIOLACEAE SPECIES

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ROSHAN AKRAM; ZARRABI MAHBOOBE; ASGARANI EZAT; KANAANI MOHAMMAD REZA

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Seminar Paper

Paper Information

Seminar: CONGRESS OF IRANIAN GENETICS
Year:2014 | Issue:1

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Title

SCREENING OF CYCLOTIDES AS ANTIMICROBIAL PEPTIDES FROM SEVERAL VIOLACEAE SPECIES

Author(s)

ROSHAN AKRAM | ZARRABI MAHBOOBE | ASGARANI EZAT | KANAANI MOHAMMAD REZA

Keywords

CYCLOTIDES

VIOLACEAE

VBC GENE

PCR

Abstract

CYCLOTIDES ARE PLANT POLYPEPTIDES CHARACTERIZED BY THEIR UNIQUE CYCLIC CYSTEINE KNOT STRUCTURAL MOTIF. IT IS THOUGHT THAT THEY FUNCTION PRIMARILY AS DEFENSE AGENTS IN PLANTS. BUT THEY ALSO HAVE VARIETY OF OTHER BIOLOGICAL PROPERTIES, INCLUDING ANTI-HIV, ANTIMICROBIAL AND CYTOTOXIC EFFECTS. BECAUSE OF THEIR EXCEPTIONAL STABILITY, THEY HAVE ATTRACTED INTEREST AS POTENTIAL PRIMING MATERIALS FOR PROTEIN ENGINEERING AND DRUG DESIGN. IN THE PRESENT STUDY, WE DETERMINED THE CYCLOTIDES CONTENT OF THE VIOLACEAE FAMILY. THE SAMPLES COLLECTED FROM ZANJAN AND TOTAL DNA WAS EXTRACTED BY CTAB BUFFER. FORWARD AND REVERSE PRIMER AGAINST CONSERVE SEQUENCE IN VBC FAMILY OF VIOLACEAE WERE DESIGNED AND USED TO DETERMINE VBC GENE FROM VIOLA IGNOBILIS, VIOLA OCCULTA AND VIOLA ODORATA BY PCR AMPLIFICATION OF THE VBC GENE. RESULTS SHOWED THE PRESENCE OF VBC GENE FOR THE FIRST TIME IN THESE SPECIES. THE SAMPLES WERE COLLECTED FROM NEARLY SAME LOCATION; THEREFORE THEY HAVE SIMILAR BIOLOGICAL PROPERTIES. IT CAN BE SUGGESTED THAT THE ENVIRONMENTAL CONDITIONS STRONGLY AFFECT ON CYCLOTIDE CONTENT OF VIOLACEAE.

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APA: Copy

ROSHAN, AKRAM, ZARRABI, MAHBOOBE, ASGARANI, EZAT, & KANAANI, MOHAMMAD REZA. (2014). SCREENING OF CYCLOTIDES AS ANTIMICROBIAL PEPTIDES FROM SEVERAL VIOLACEAE SPECIES. CONGRESS OF IRANIAN GENETICS. SID. https://sid.ir/paper/906877/en

Vancouver: Copy

ROSHAN AKRAM, ZARRABI MAHBOOBE, ASGARANI EZAT, KANAANI MOHAMMAD REZA. SCREENING OF CYCLOTIDES AS ANTIMICROBIAL PEPTIDES FROM SEVERAL VIOLACEAE SPECIES. 2014. Available from: https://sid.ir/paper/906877/en

IEEE: Copy

AKRAM ROSHAN, MAHBOOBE ZARRABI, EZAT ASGARANI, and MOHAMMAD REZA KANAANI, “SCREENING OF CYCLOTIDES AS ANTIMICROBIAL PEPTIDES FROM SEVERAL VIOLACEAE SPECIES,” presented at the CONGRESS OF IRANIAN GENETICS. 2014, [Online]. Available: https://sid.ir/paper/906877/en

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