USE OF PCR-RFLP FOR ASSESSMENT OF GENETIC DIVERSITY AMONG XANTHOMONAS SPP.

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GHASHGHAEI TAHEREH; ASGARANI EZAT; SOUDI MOHAMMAD REZA; SALMANZADEH AHRABI SIAVASH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Seminar Paper

Paper Information

Seminar: CONGRESS OF IRANIAN GENETICS
Year:2014 | Issue:1

Download Full-Text

View:

204

Download:

Information Seminar Paper

Title

USE OF PCR-RFLP FOR ASSESSMENT OF GENETIC DIVERSITY AMONG XANTHOMONAS SPP.

Author(s)

GHASHGHAEI TAHEREH | ASGARANI EZAT | SOUDI MOHAMMAD REZA | SALMANZADEH AHRABI SIAVASH

Keywords

PCR- RFLP

XANTHOMONAS SPP.

BIODIVERSITY

Abstract

THE GENUS XANTHOMONAS IS FORMED BY A COMPLEX GROUP OF BACTERIAL SPECIES WITH DIVERSE PHYSIOLOGICAL AND PHYTOPATHOLOGICAL TRAITS. THIS GROUP OF PHYTOPATHOGENS INFECTS MANY ECONOMICALLY IMPORTANT PLANTS SUCH AS COTTON, BEANS, RICE, CRUCIFERS AND THEREFORE, THESE BACTERIA REPRESENT A THREAT TO AGRICULTURE AROUND THE WORLD. SOME OF THE MOST IMPORTANT DISEASES CAUSED BY XANTHOMONAS SPECIES INCLUDE CITRUS CANKER AND BLACK ROT, ADDITIONALLY XANTHOMONAS IS ALSO IMPORTANT IN BIOTECHNOLOGY DUE TO THE PRODUCTION OF A POLYSACCHARIDE KNOWN AS A XANTHAN GUM, WHICH IS USED AS A STABILIZER AND EMULSIFIER IN THE FOOD AND COSMETIC INDUSTRY, AMONG OTHER APPLICATIONS. IN THIS STUDY GENETIC DIVERSITY AMONG 15 STRAINS OF XANTHOMONAS SPP. WERE CLARIFIED BY PCR RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) ANALYSIS OF 16S-23S RDNA INTERNAL TRANSCRIBED SPACER (ITS) REGION USING FOUR RESTRICTION ENZYMES INCLUDING ALUI, MBOI, DDEI, BSNI. TOTAL BACTERIAL DNA WAS PREPARED BY USING PHENOL- CHLOROFORM METHOD. PCR WAS PERFORMED BY USING ITS PRIMER, PCR PRODUCTS WERE DIGESTED WITH RESTRICTION ENZYMES. THREE ENZYMES INCLUDING ALUI, DDEI, BSNI SHOWED TWO DIFFERENT PROFILES AMONG ALL STRAINS BUT ONE ENZYME, MBOI, SHOWED THREE DIFFERENT PROFILES. PCR- RFLP ANALYSIS OF IT’S IS A SIMPLE AND RAPID TECHNIQUE TO EVALUATE BIODIVERSITY AMONG XANTHOMONAS SPP. AND CAN USE FOR PARTIALLY DIFFERENTIATION AMONG XANTHOMONAS SPECIES.

Cites

No record.

References

No record.

Cite

APA: Copy

GHASHGHAEI, TAHEREH, ASGARANI, EZAT, SOUDI, MOHAMMAD REZA, & SALMANZADEH AHRABI, SIAVASH. (2014). USE OF PCR-RFLP FOR ASSESSMENT OF GENETIC DIVERSITY AMONG XANTHOMONAS SPP.. CONGRESS OF IRANIAN GENETICS. SID. https://sid.ir/paper/906881/en

Vancouver: Copy

GHASHGHAEI TAHEREH, ASGARANI EZAT, SOUDI MOHAMMAD REZA, SALMANZADEH AHRABI SIAVASH. USE OF PCR-RFLP FOR ASSESSMENT OF GENETIC DIVERSITY AMONG XANTHOMONAS SPP.. 2014. Available from: https://sid.ir/paper/906881/en

IEEE: Copy

TAHEREH GHASHGHAEI, EZAT ASGARANI, MOHAMMAD REZA SOUDI, and SIAVASH SALMANZADEH AHRABI, “USE OF PCR-RFLP FOR ASSESSMENT OF GENETIC DIVERSITY AMONG XANTHOMONAS SPP.,” presented at the CONGRESS OF IRANIAN GENETICS. 2014, [Online]. Available: https://sid.ir/paper/906881/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops