DETECTION OF A DNA SEQUENCE RELATED TO HEPATITIS B VIRUS WITH A SENSITIVE ELECTROCHEMICAL DNA BIOSENSOR CONSTRUCTED WITH FUNCTIONALIZING OF MAGNETITE NANOPARTICLES BY 4-NITROBENZENEDIAZONIUM

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MASHHADIZADEH MOHAMMAD HOSSEIN; POURTAGHAVI TALEMI RASOUL

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Seminar Paper

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Seminar: IRANIAN SEMINAR OF ANALYTICAL CHEMISTRY
Year:2014 | Issue:20

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Title

DETECTION OF A DNA SEQUENCE RELATED TO HEPATITIS B VIRUS WITH A SENSITIVE ELECTROCHEMICAL DNA BIOSENSOR CONSTRUCTED WITH FUNCTIONALIZING OF MAGNETITE NANOPARTICLES BY 4-NITROBENZENEDIAZONIUM

Author(s)

MASHHADIZADEH MOHAMMAD HOSSEIN | POURTAGHAVI TALEMI RASOUL | Issue Writer Certificate

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Abstract

IN RECENT YEARS, THE INTEREST FOR DNA-BASED DIAGNOSTIC TESTS HAS BEEN GROWING. THE DEVELOPMENT OF SYSTEMS ALLOWING DNA DETECTION IS MOTIVATED BY APPLICATIONS IN MANY FIELDS: DNA DIAGNOSTICS, GENE ANALYSIS, FAST DETECTION OF BIOLOGICAL WARFARE AGENTS, AND FORENSIC APPLICATIONS.DETECTION OF GENETIC MUTATIONS AT THE MOLECULAR LEVEL OPENS UP THE POSSIBILITY OF PERFORMING RELIABLE DIAGNOSTICS EVEN BEFORE ANY SYMPTOM OF A DISEASE APPEARS. OPTICAL [1], ELECTROCHEMICAL [2], AND MICRO-GRAVIMETRICAL [3] DNA TRANSDUCTIONS HAVE BEEN WIDELY STUDIED. AN IN SITU METHOD FOR DIRECT ATTACHMENT OF 4-NITROBENZENEDIAZONIUM ONTO THE SURFACE OF MAGNETITE NANOPARTICLES TO DEVELOP AN EFFICIENT GUIDE FOR CONSTRUCTION OF DNA ELECTROCHEMICAL BIOSENSOR WAS PROPOSED. FOR FUNCTIONALIZATION OF MAGNETIC NANOPARTICLES, FIRSTLY AZO SALT OF NITROANILINE (4-NITROBENZENEDIAZONIUM) WAS PREPARED AND WAS REACTED DIRECTLY WITH MAGNETITE NANOPARTICLES IN BASIC SOLUTION FOLLOWED BY ELECTROCHEMICAL REDUCTION OF THE TERMINAL NITRO GROUP TO AMINE. THIS NH2 GROUP CAN INTERACT WITH AN ACTIVATED PHOSPHATE GROUP OF NON-LABELED SS-DNA RELATED TO HEPATITIS B VIRUS. ACTIVATED NON-LABELED SS-DNA WAS PREPARED USINGN - (3 DIMETHYLAMINOPROPYL) -N -ETHYL-CARBODIIMIDE HYDROCHLORIDE (EDC) AND N -HYDROXY-SUCCINIMIDE (NHS). THE DNA HYBRIDIZATION EVENTS WERE MONITORED BY DIFFERENTIAL PULSE VOLTAMMETRY (DPV) MEASUREMENT OF THE INTERCALATED METHYLENE BLUE (MB) AS A WELL-KNOWN ELECTROCHEMICAL INDICATOR. UNDER OPTIMAL CONDITIONS, THE REDUCTION PEAK CURRENTS WERE LINEARLY DECREASED WITH INCREASING THE CONCENTRATION OF HBV TARGET DNA FROM 7.8 (±0.2) × 10-13 MOL L-1 TO 8.8 (±0.3) × 10-9 MOL L-1 WITH A DETECTION LIMIT OF 3.3 (±0.2) × 10-13 MOL L-1. THIS DNA BIOSENSOR EXHIBITED EXCELLENT SELECTIVITY, REPRODUCIBILITY, AND STABILITY IN THE HBV DNA HYBRIDIZATION ASSAY.

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APA: Copy

MASHHADIZADEH, MOHAMMAD HOSSEIN, & POURTAGHAVI TALEMI, RASOUL. (2014). DETECTION OF A DNA SEQUENCE RELATED TO HEPATITIS B VIRUS WITH A SENSITIVE ELECTROCHEMICAL DNA BIOSENSOR CONSTRUCTED WITH FUNCTIONALIZING OF MAGNETITE NANOPARTICLES BY 4-NITROBENZENEDIAZONIUM. IRANIAN SEMINAR OF ANALYTICAL CHEMISTRY. SID. https://sid.ir/paper/911724/en

Vancouver: Copy

MASHHADIZADEH MOHAMMAD HOSSEIN, POURTAGHAVI TALEMI RASOUL. DETECTION OF A DNA SEQUENCE RELATED TO HEPATITIS B VIRUS WITH A SENSITIVE ELECTROCHEMICAL DNA BIOSENSOR CONSTRUCTED WITH FUNCTIONALIZING OF MAGNETITE NANOPARTICLES BY 4-NITROBENZENEDIAZONIUM. 2014. Available from: https://sid.ir/paper/911724/en

IEEE: Copy

MOHAMMAD HOSSEIN MASHHADIZADEH, and RASOUL POURTAGHAVI TALEMI, “DETECTION OF A DNA SEQUENCE RELATED TO HEPATITIS B VIRUS WITH A SENSITIVE ELECTROCHEMICAL DNA BIOSENSOR CONSTRUCTED WITH FUNCTIONALIZING OF MAGNETITE NANOPARTICLES BY 4-NITROBENZENEDIAZONIUM,” presented at the IRANIAN SEMINAR OF ANALYTICAL CHEMISTRY. 2014, [Online]. Available: https://sid.ir/paper/911724/en

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