ON/OFF-SWITCHABLE MOLECULARLY IMPRINTED FOLIC ACID “PLASTIC” SENSOR

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KARIMIAN NAJMEH; ARBAB ZAVAR MOHAMMAD HOSSEIN; CHAMSAZ MAHMOUD; F.TURNER ANTHONY P.; TIWARI ASHUTOSH

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Seminar Paper

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Seminar: IRANIAN BIENNIAL ELECTROCHEMISTRY SEMINAR
Year:2014 | Issue:11

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Title

ON/OFF-SWITCHABLE MOLECULARLY IMPRINTED FOLIC ACID “PLASTIC” SENSOR

Author(s)

Keywords

MOLECULARLY IMPRINTING POLYMER

SMART POLYMERS

ON/OFF SWITCHABLE FOLIC ACID SENSOR REFERENCES

Abstract

MOLECULAR IMPRINTING OF POLYMERS (MIP) IS A TECHNIQUE USED TO CREATE PLASTIC RECEPTORS BY THE FORMATION OF A POLYMER NETWORK AROUND A TEMPLATE MOLECULE. MIPS HAVE GREAT POTENTIAL IN BIOANALYTICS DUE TO THEIR EASE OF PREPARATION, LOW COST AND A HIGH SENSITIVITY, SELECTIVITY AND STABILITY. THE ELECTROSYNTHETIC APPROACH DUE TO CREATE SIMPLY AND RAPIDLY AN ADHERENT AND COMPACT POLYMERIC FILM WITH CONTROLLABLE THICKNESS AND DIRECT COMMUNICATION BETWEEN THE POLYMER AND THE SURFACE OF THE TRANSDUCER COULD BE VERY HELPFUL BOTH IN IMPROVING THE MOLECULAR IMPRINTING POLYMERIZATION (MIP) PROCEDURE AND IN EXTENDING THE APPLICATION OF MIP [1-2]. THE COMBINATION OF SMART POLYMERS WITH MOLECULAR IMPRINTING OFFERS A POWERFUL TOOL TO DESIGN MORE EFFECTIVE SENSORS AND MEDICAL DEVICES [3]. IN THIS WORK, A TEMPERATURE SENSITIVE AMINE-TERMINATED POLY(N-ISOPROPYLACRYLAMIDE) BLOCK WITH (N,N’-METHYLENEBISACRYLAMIDE) CROSS-LINKER ALONG WITH OPHENYLENEDIAMINE WAS ELECTROPOLYMERISED ON A GOLD ELECTRODE IN THE PRESENCE OF FOLIC ACID (FA) AS TEMPLATE TO PRODUCE AN ON/OFF-SWITCHABLE MOLECULARLY IMPRINTED POLYMER (MIP) AFFINITY SENSOR FOR FOLIC ACID. THE TEMPLATE MOLECULES WERE REMOVED FROM THE MIP ELECTRODE SURFACE BY WASHING WITH A METHANOLACETIC ACID (9:1, V/V) SOLUTION. DIFFERENTIAL PULSE VOLTAMMETRY AND CYCLIC VOLTAMMETRY WERE USED TO CHARACTERISE THE FA-IMPRINTED LAYER. INCUBATION OF THE MIP-MODIFIED ELECTRODE WITH FA RESULTED IN A SUPPRESSION OF THE FERRO/FERRICYANIDE REDOX PROCESS. THE HIGHEST SENSITIVITY OF THIS TEMPERATURE GATED ON/OFF SWITCHABLE FOLIC ACID SENSOR WAS ACHIEVED AT 22OC. SUCH SWITCHABLE AFFINITY MATERIALS OFFER CONSIDERABLE POTENTIAL FOR THE DESIGN OF HIGHLY SELECTIVE AND CONTROLLABLE BIOSENSORS AND IMMUNOASSAYS.

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APA: Copy

KARIMIAN, NAJMEH, ARBAB ZAVAR, MOHAMMAD HOSSEIN, CHAMSAZ, MAHMOUD, F.TURNER, ANTHONY P., & TIWARI, ASHUTOSH. (2014). ON/OFF-SWITCHABLE MOLECULARLY IMPRINTED FOLIC ACID “PLASTIC” SENSOR. IRANIAN BIENNIAL ELECTROCHEMISTRY SEMINAR. SID. https://sid.ir/paper/912086/en

Vancouver: Copy

KARIMIAN NAJMEH, ARBAB ZAVAR MOHAMMAD HOSSEIN, CHAMSAZ MAHMOUD, F.TURNER ANTHONY P., TIWARI ASHUTOSH. ON/OFF-SWITCHABLE MOLECULARLY IMPRINTED FOLIC ACID “PLASTIC” SENSOR. 2014. Available from: https://sid.ir/paper/912086/en

IEEE: Copy

NAJMEH KARIMIAN, MOHAMMAD HOSSEIN ARBAB ZAVAR, MAHMOUD CHAMSAZ, ANTHONY P. F.TURNER, and ASHUTOSH TIWARI, “ON/OFF-SWITCHABLE MOLECULARLY IMPRINTED FOLIC ACID “PLASTIC” SENSOR,” presented at the IRANIAN BIENNIAL ELECTROCHEMISTRY SEMINAR. 2014, [Online]. Available: https://sid.ir/paper/912086/en

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