INFLUENCE OF CERIUM (III) CHLORIDE ON FOLDING PATHWAY OF HUMAN SERUM ALBUMIN

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SHALBAFAN M.; GHEIBI N.; REZAEI BEHBEHANI G.

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Seminar: IRANIAN CHEMISTRY CONGRESS
Year:2013 | Issue:16

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Title

INFLUENCE OF CERIUM (III) CHLORIDE ON FOLDING PATHWAY OF HUMAN SERUM ALBUMIN

Author(s)

SHALBAFAN M. | GHEIBI N. | REZAEI BEHBEHANI G. | Issue Writer Certificate

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Abstract

IN ORDER TO UNDERSTAND THE EFFECT OF CERIUM ION ON THE STRUCTURAL STABILITY OF PROTEINS, UREA- INDUCED UNFOLDING TRANSITION OF HUMAN SERUM ALBUMIN (HSA) IS CHARACTERIZED BY UV- VISIBLE SPECTRA. SOLUTIONS FOR EXPERIMENTS WERE PREPARED FROM STOCK SOLUTIONS OF PROTEIN AND SALT (CECL3) PREPARED IN PHOSPHATE BUFFER (PH7.4). THE CONCENTRATION OF PROTEIN AND SALT WERE 2 MM AND 64 MM, RESPECTIVELY. THE COMPLEX SOLUTIONS WERE INCUBATED FOR ENOUGH TIME (>6 H) AT 25°C. ON THE CHEMICAL DENATURATION EXPERIMENTS, TO A STOCK PROTEIN SOLUTION, DIFFERENT VOLUMES OF UREA SOLUTION (6M) WERE ADDED TO OBTAIN THE DESIRED CONCENTRATION OF DENATURANT. OUR RESULTS PROVIDE EVIDENCE THAT THE CERIUM IONALTER THE UREA-INDUCED UNFOLDING PATHWAY OF HSA. FURTHERMORE, THE CHANGE OF FREE ENERGY (DGH2O), THE MIDPOINT OF CHEMICAL DENATURATION (CM) AND THE SOLVENT- EXPOSED SURFACE (M) DURING THE UREA- INDUCED UNFOLDING PROCESS HAVE ALSO BEEN OBTAINED TO CHARACTERIZE THE EFFECTS OF CERIUM ION ON THE CONFORMATIONAL STABILITY OF HAS [1-4]. THE VALUE OF M IS INCREASED IN THE PRESENCE OF CERIUM ION, INDICATING THAT THE SOLVENT-EXPOSED SURFACE OF THE HSA MOLECULES IN THE UNFOLDING PROCESS IS MORE THAN THAT OF HSA WITHOUT LANTHANUM ION. THE CM AND DGH2O FOR THE HAS WITH CE3+ ION[CM =1.90/M AND DGH2O =7.78 KJ.M-1 RESPECTIVELY] IN THE UNFOLDING PROCESS ARE ALL INCREASED WITH RESPECT TO THAT OF HSA WITHOUT CERIUM ION [CM.=1.58/M AND DGH2O=7.02 KJ.M-1] THIS IS SHOWN THAT THIS ION UNSTABLE TERTIARY STRUCTURE OF HSA. INSTABILITY OF HSA STRUCTURE IS A BAD SIDE EFFECT OF USING CE3+ COMPOUNDS AS ANTI EMETING DRUG. [5].

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APA: Copy

SHALBAFAN, M., GHEIBI, N., & REZAEI BEHBEHANI, G.. (2013). INFLUENCE OF CERIUM (III) CHLORIDE ON FOLDING PATHWAY OF HUMAN SERUM ALBUMIN. IRANIAN CHEMISTRY CONGRESS. SID. https://sid.ir/paper/915701/en

Vancouver: Copy

SHALBAFAN M., GHEIBI N., REZAEI BEHBEHANI G.. INFLUENCE OF CERIUM (III) CHLORIDE ON FOLDING PATHWAY OF HUMAN SERUM ALBUMIN. 2013. Available from: https://sid.ir/paper/915701/en

IEEE: Copy

M. SHALBAFAN, N. GHEIBI, and G. REZAEI BEHBEHANI, “INFLUENCE OF CERIUM (III) CHLORIDE ON FOLDING PATHWAY OF HUMAN SERUM ALBUMIN,” presented at the IRANIAN CHEMISTRY CONGRESS. 2013, [Online]. Available: https://sid.ir/paper/915701/en

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