COMPARISON OF DIFFERENT METHODS FOR DNA EXTRACTION FROM SOIL SAMPLES

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TEIMOURI MARYAM; MOHAMMADI PARISA; ASGARANI EZZAT; JALILI ADEL; Gholipour Mahnaz

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Seminar Paper

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Seminar: INTERNATIONAL AND IRANIAN CONGRESS OF MICROBIOLOGY
Year:2015 | Issue:16

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Title

COMPARISON OF DIFFERENT METHODS FOR DNA EXTRACTION FROM SOIL SAMPLES

Author(s)

Keywords

DNA

SOIL

SDS

CTAB

PVP

Abstract

BACKGROUND AND AIM: SOIL CONSISTS OF A SET OF DIFFERENT SUBSTANCES, GASES, WATER AND MANY ORGANISMS THAT TOGETHER SUPPORT ANY FORM OF LIFE. TO STUDY MICROBIAL DIVERSITY AND ITS FUNCTION IN SOIL ECOSYSTEM, IT IS NECESSARY TO USE CLASSIC AND MOLECULAR TECHNIQUES.NOWADAYS, ENVIRONMENTAL MICROBIOLOGISTS USE MOLECULAR TECHNIQUES RATHER THAN TRADITIONAL METHODS TO STUDY SOIL MICROBIAL COMMUNITIES. THE FIRST STEP IN MOLECULAR ANALYSES OF SOIL IS EXTRACTION OF DNA. BECAUSE OF THE PRESENCE OF MANY COMPONENTS AND MATERIALS IN A SOIL SAMPLE, EXTRACTION OF PURE DNA IS DIFFICULT. THE OBJECTIVE OF THIS STUDY WAS TO EXTRACT DNA FROM SOILSAMPLES BY FOUR DIFFERENT METHODS AND COMPARE THEIR EFFICIENCY....

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APA: Copy

TEIMOURI, MARYAM, MOHAMMADI, PARISA, ASGARANI, EZZAT, JALILI, ADEL, & Gholipour, Mahnaz. (2015). COMPARISON OF DIFFERENT METHODS FOR DNA EXTRACTION FROM SOIL SAMPLES. INTERNATIONAL AND IRANIAN CONGRESS OF MICROBIOLOGY. SID. https://sid.ir/paper/925757/en

Vancouver: Copy

TEIMOURI MARYAM, MOHAMMADI PARISA, ASGARANI EZZAT, JALILI ADEL, Gholipour Mahnaz. COMPARISON OF DIFFERENT METHODS FOR DNA EXTRACTION FROM SOIL SAMPLES. 2015. Available from: https://sid.ir/paper/925757/en

IEEE: Copy

MARYAM TEIMOURI, PARISA MOHAMMADI, EZZAT ASGARANI, ADEL JALILI, and Mahnaz Gholipour, “COMPARISON OF DIFFERENT METHODS FOR DNA EXTRACTION FROM SOIL SAMPLES,” presented at the INTERNATIONAL AND IRANIAN CONGRESS OF MICROBIOLOGY. 2015, [Online]. Available: https://sid.ir/paper/925757/en

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