INVESTIGATION OF CATALYTIC ACTIVATION OF A BACILLUS LACCASE AFTER TEMPERATURE TREATMENT

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MOLLANIA NASRIN; Fathi Roudsari Mehrnoosh; KHAJEH KHOSRO

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Seminar Paper

Paper Information

Seminar: INTERNATIONAL IRANIAN BIOLOGY CONFERENCE
Year:2012 | Issue:17

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Title

INVESTIGATION OF CATALYTIC ACTIVATION OF A BACILLUS LACCASE AFTER TEMPERATURE TREATMENT

Author(s)

MOLLANIA NASRIN | Fathi Roudsari Mehrnoosh | KHAJEH KHOSRO

Keywords

LACCASE

INTRINSIC FLUORESCENCE

GAS-PHASE ELECTROPHORETIC MOBILITY MACROMOLECULE ANALYSIS

THERMOACTIVATION

Abstract

LACCASES BELONG TO A FAMILY OF MULTICOPPER OXIDASES THAT HAVE STRONG OXIDATION ABILITIES TOWARDS PHENOLIC COMPOUNDS. INTERESTINGLY, NEW ISOLATED LACCASE SHOWED THE REMARKABLE BEHAVIOR OF ACTIVATION AFTER THERMAL TREATMENT. THE ACTIVITY INCREASED AT LEAST 300% ALSO THE KCAT WAS SUCCESSFULLY ENHANCED (2.5 FOLD) AFTER 50 MIN INCUBATION AT 70OC. DUE TO THE LIMITED KNOWLEDGE PRESENTED ON THIS INTERESTING PHENOMENON, THE PRESENT PAPER CONCERNS THE DIFFERENCES WHICH ARE INDUCED IN ENZYME STRUCTURE AFTER THERMAL TREATMENT.THE LOCAL CHANGES IN SECONDARY STRUCTURES WERE ALSO OBSEVED. OLIGOMERIZATION STUDIES OF THE ENZYME USING GAS-PHASE ELECTROPHORETIC MOBILITY MACROMOLECULE ANALYSIS (GEMMA) DID NOT PROVE ANY OLIGOMERIZATION.

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APA: Copy

MOLLANIA, NASRIN, Fathi Roudsari, Mehrnoosh, & KHAJEH, KHOSRO. (2012). INVESTIGATION OF CATALYTIC ACTIVATION OF A BACILLUS LACCASE AFTER TEMPERATURE TREATMENT. INTERNATIONAL IRANIAN BIOLOGY CONFERENCE. SID. https://sid.ir/paper/929099/en

Vancouver: Copy

MOLLANIA NASRIN, Fathi Roudsari Mehrnoosh, KHAJEH KHOSRO. INVESTIGATION OF CATALYTIC ACTIVATION OF A BACILLUS LACCASE AFTER TEMPERATURE TREATMENT. 2012. Available from: https://sid.ir/paper/929099/en

IEEE: Copy

NASRIN MOLLANIA, Mehrnoosh Fathi Roudsari, and KHOSRO KHAJEH, “INVESTIGATION OF CATALYTIC ACTIVATION OF A BACILLUS LACCASE AFTER TEMPERATURE TREATMENT,” presented at the INTERNATIONAL IRANIAN BIOLOGY CONFERENCE. 2012, [Online]. Available: https://sid.ir/paper/929099/en

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