MULTIPLEX-PCR DETECTION OF FUMONISIN-PRODUCING F.VERTICILLIOIDES AND F.PROLIFERATUM IN CORN OF FEEDSTUFF

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Mehrzad Asiyeh; MORTAZAVI SEYED ALI; Mehraban Sangatash Masumeh; Rezaeian Doloei Roya; Mohsenzadeh Mohammad

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Seminar Paper

Paper Information

Seminar: INTERNATIONAL CONFERENCE ON MODERN RESEARCH IN AGRICULTURAL SCIENCE AND ENVIRONMENT
Year:2015 | Issue:1

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Title

MULTIPLEX-PCR DETECTION OF FUMONISIN-PRODUCING F.VERTICILLIOIDES AND F.PROLIFERATUM IN CORN OF FEEDSTUFF

Author(s)

Mehrzad Asiyeh | MORTAZAVI SEYED ALI | Mehraban Sangatash Masumeh | Rezaeian Doloei Roya | Mohsenzadeh Mohammad

Keywords

CORN

MULTIPLEX-PCR

F. VERTICILLIOIDES

F. PROLIFERATUM

FUMONISIN

Abstract

CEREALS CONTAMINATION OF FEEDSTUFF SUCH AS CORN WITH MYCOTOXIGENIC SPECIES OF FUSARIUM IS CONSIDERED AS A MAIN SOURCE OF FUMONISIN MYCOTOXIN GROUPS WHICH CAUSE WORLDWIDE SERIOUS DISEASES IN HUMAN AND ANIMAL HEALTH. ACCORDING, IN THIS STUDY, A TOTAL OF 100 FUSARIUM ISOLATES WERE RECOVERED FROM CORN SAMPLES COLLECTED FROM 16 DAIRY FARMS IN RAZAVY KHORASAN PROVINCE, IRAN, DURING 2011-2012. FUSARIUM ISOLATES WERE IDENTIFIED ON MORPHOLOGICAL AND USING SPECIES-SPECIFIC PCR. CONSIDERING THAT, EARLY DETECTION OF FUSARIUM SPECIES COULD BE VERY IMPORTANT TO PREVENT MYCOTOXIN CONTAMINATING FOOD AND FEED GRAINS, A MULTIPLEX-PCR ASSAY WAS DESIGNED ON THE BASE OF FOUR SETS OF SPECIFIC PRIMERS INCLUDING: ITS-F/R, VERT1/2, FUM1-F/R AND FP3-F/FP4-R WHICH HAS BEEN DEVELOPED FOR THE DISCOVERY OF THE TWO COMMON FUSARIUM SPECIES (F. VERTICILLIOIDES AND F. PROLIFERATUM) THAT THEY CAN POTENTIALLY PRODUCE FUMONISIN. IN THIS STUDY, PCR ANALYSIS COMFIRMED 78% OF MORPHOLOGICAL IDENTIFICATION TO SPECIES LEVEL AS F. PROLIFERATUM (53.8%) AND 29.5% AS F. VERTICILLIOIDES. 42 (53.84%) OF TOTAL (78) F. VERTICILLIOIDES AND F. PROLIFERATUM ISOLATED WERE FUMONISIN PRODUCING OUT OF WHICH 24 (57.14%) WERE F. PROLIFERATUM WHILE 18 (42.86 %) WERE F. VERTICILLIOIDES. MOREOVER, WE HAVE ESTABLISHED THE METHOD TO DISCRIMINATE BETWEEN THE TWO POPULATIONS F. VERTICILLIOIDES AND F. PROLIFERATUM, SIMULTANEOUSLY ENABLE US TO DISCRIMINATE FUMONISIN-PRODUCING STRAINS FROM NON-FUMONISIN-PRODUCING STRAINS IN THE CORN GRAINS OF ANIMAL FEEDSTUFF.

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APA: Copy

Mehrzad, Asiyeh, MORTAZAVI, SEYED ALI, Mehraban Sangatash, Masumeh, Rezaeian Doloei, Roya, & Mohsenzadeh, Mohammad. (2015). MULTIPLEX-PCR DETECTION OF FUMONISIN-PRODUCING F.VERTICILLIOIDES AND F.PROLIFERATUM IN CORN OF FEEDSTUFF. INTERNATIONAL CONFERENCE ON MODERN RESEARCH IN AGRICULTURAL SCIENCE AND ENVIRONMENT. SID. https://sid.ir/paper/933396/en

Vancouver: Copy

Mehrzad Asiyeh, MORTAZAVI SEYED ALI, Mehraban Sangatash Masumeh, Rezaeian Doloei Roya, Mohsenzadeh Mohammad. MULTIPLEX-PCR DETECTION OF FUMONISIN-PRODUCING F.VERTICILLIOIDES AND F.PROLIFERATUM IN CORN OF FEEDSTUFF. 2015. Available from: https://sid.ir/paper/933396/en

IEEE: Copy

Asiyeh Mehrzad, SEYED ALI MORTAZAVI, Masumeh Mehraban Sangatash, Roya Rezaeian Doloei, and Mohammad Mohsenzadeh, “MULTIPLEX-PCR DETECTION OF FUMONISIN-PRODUCING F.VERTICILLIOIDES AND F.PROLIFERATUM IN CORN OF FEEDSTUFF,” presented at the INTERNATIONAL CONFERENCE ON MODERN RESEARCH IN AGRICULTURAL SCIENCE AND ENVIRONMENT. 2015, [Online]. Available: https://sid.ir/paper/933396/en

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