EVALUATION OF GENETIC DIVERSITY OF SUGARCANE VARIANTS USING MORPHOLOGICAL CHARACTERS AND RAPD MOLECULAR MARKERS

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MOHAMMADI ALI; Ghalandari Ruhollah; Alizadeh Ghorban Ali; Batat shooshtari Ali; KHATAMI MOHAMMADREZA; BARAT SHOOSHTARI MOHAMMAD

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Seminar Paper

Paper Information

Seminar: INTERNATIONAL CONFERENCE ON MODERN RESEARCH IN AGRICULTURAL SCIENCE AND ENVIRONMENT
Year:2015 | Issue:1

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Title

EVALUATION OF GENETIC DIVERSITY OF SUGARCANE VARIANTS USING MORPHOLOGICAL CHARACTERS AND RAPD MOLECULAR MARKERS

Author(s)

Keywords

SUGARCANE

RAPD MOLECULAR MARKER

GENETIC VARIATION

MORPHOLOGICAL CHARACTERISTICS

Abstract

THIS STUDY WAS ACCOMPLISHED TO ANALYZE THE GENETIC VARIATION OF 35 AGRICULTURAL SUGARCANES VARIANTS USING RAPD TECHNIQUE. THE AIM WAS TO DETERMINE THE GENETIC VARIATION LEVEL AND RELATION AMONG SOME COMMERCIAL VARIETIES OF SACCHARUM OFFICINARUM AND THE TWO WILD VARIETIES OF SACCHARUM SPONTNEUM AS WELL. TOTAL GENOMIC DNA OF EACH SAMPLE WAS EXTRACTED FROM 1.5-2.5 GR OF NEW GERMINATED FRESH YOUNG LEAVES USING MODIFIED CTAB METHOD. THE CONCENTRATION WAS DETERMINED BY SPECTROPHOTOMETER. RAPD ANALYSIS WAS PERFORMED BY USE OF 10 BP RANDOMIZED NUCLEOTIDE PRIMERS. ALL GENOTYPES WERE STUDIED FOR PRESENCE OR ABSENCE OF BANDS, AND ANALYZED BY USE OF STATISTICAL SOFTWARE NTSYS 2002. AMONG 65 USED PRIMERS, 30 OF THEM SHOWED POLYMORPHISM BETWEEN THE SAMPLE, AND 462 POLYMORPHIC BANDS WERE PRODUCED. CLUSTER ANALYSIS LAYS VARIETIES INTO TWO SEPARATED GROUPS THROUGH THE JACARD COEFFICIENT AND UPGMA METHODS. IN THIS CLASSIFICATION, THE TWO WILD VARIETIES FROM THE SACCHARUM SPONTANENM WERE SEPARATED COMPLETELY AND SHOWED COMPLETELY DIFFERENT BAND PATTERN IN COMPARISON WITH OTHER VARIETIES. GENERALLY, THE RESULTS OF THIS RESEARCH SHOWED THAT RAPD MARKER IS A SUITABLE TOOL TO STUDY GENETIC VARIATION IN SUGARCANE. IT IS RECOMMENDED TO INCREASE THE EFFICIENCY OF BREEDING PROGRAMS TO STUDY PRECISE GENETIC VARIATION AND TO DETERMINE THE RELATIONSHIP AMONG CULTIVARS. OVERALL, THE RESULTS INDICATED THAT RAPD TECHNIQUE IN COMBINATION WITH MORPHOLOGICAL SELECTION AND IDENTIFICATION COULD SERVE AS AN IDEAL TOOL FOR STUDYING THE GENETIC VARIATION AMONG SUGARCANE FAMILIES.

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APA: Copy

MOHAMMADI, ALI, Ghalandari, Ruhollah, Alizadeh, Ghorban Ali, Batat shooshtari, Ali, KHATAMI, MOHAMMADREZA, & BARAT SHOOSHTARI, MOHAMMAD. (2015). EVALUATION OF GENETIC DIVERSITY OF SUGARCANE VARIANTS USING MORPHOLOGICAL CHARACTERS AND RAPD MOLECULAR MARKERS. INTERNATIONAL CONFERENCE ON MODERN RESEARCH IN AGRICULTURAL SCIENCE AND ENVIRONMENT. SID. https://sid.ir/paper/933554/en

Vancouver: Copy

MOHAMMADI ALI, Ghalandari Ruhollah, Alizadeh Ghorban Ali, Batat shooshtari Ali, KHATAMI MOHAMMADREZA, BARAT SHOOSHTARI MOHAMMAD. EVALUATION OF GENETIC DIVERSITY OF SUGARCANE VARIANTS USING MORPHOLOGICAL CHARACTERS AND RAPD MOLECULAR MARKERS. 2015. Available from: https://sid.ir/paper/933554/en

IEEE: Copy

ALI MOHAMMADI, Ruhollah Ghalandari, Ghorban Ali Alizadeh, Ali Batat shooshtari, MOHAMMADREZA KHATAMI, and MOHAMMAD BARAT SHOOSHTARI, “EVALUATION OF GENETIC DIVERSITY OF SUGARCANE VARIANTS USING MORPHOLOGICAL CHARACTERS AND RAPD MOLECULAR MARKERS,” presented at the INTERNATIONAL CONFERENCE ON MODERN RESEARCH IN AGRICULTURAL SCIENCE AND ENVIRONMENT. 2015, [Online]. Available: https://sid.ir/paper/933554/en

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