The Effect of Culture Medium, Organic Matter and Salinity on the Amount and Active Ingredients of Lemon Balm (Melissa officinalis L. ) Essential Oil

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Rahmanian Shima; ABOUTALEBI JAHROMI ABDOLHOSSEIN; HOSSEINI FARAHI MEHDI

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Journal Paper

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Journal: SUSTAINABLE AGRICULTURAL SCIENCE RESEARCH Year:2022 | Volume:1 | Issue:4 Page(s): 45-63

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Title

The Effect of Culture Medium, Organic Matter and Salinity on the Amount and Active Ingredients of Lemon Balm (Melissa officinalis L. ) Essential Oil

Author(s)

Rahmanian Shima | ABOUTALEBI JAHROMI ABDOLHOSSEIN | HOSSEINI FARAHI MEHDI | Issue Writer Certificate

Keywords

Compost

Vermicompost

Sodium chloride

Essential oil compounds

Abstract

To study the effect of culture medium, organic matter, and salinity on active compounds of lemon balm, a factorial experiment was conducted in completely randomized design with 3 replications. First factor (culture medium at three levels; Compost+arable soil, vermiCompost+arable soil, and Compost+vermiCompost+arable soil), second factor (organic matter; humic acid (HA) and effective microorganisms (EM) both 5 per 1000); and third factor (salinity; tap water, 40 and 80mM). Examination of essential oils by the GC method revealed the presence of 34 compounds. These compounds were affected by substrate and salinity. The major constituents of essential oils including Trans-carveol, Carvacrol acetate, Isoborneol, Isopulegol and γ-3-Carene were observed in the combined substrate of arable soil+Compost+vermiCompost, and the highest levels of 1, 3, 8-P-menthatriene, Citronellol, and γ-Terpinene were observed in the combined substrate of arable soil+Compost. With increasing salinity, amount of Trans-carveol, Carvacrol acetate, γ-Terpinene, Isoborneol, Citronellol increased, and γ-3-Carene, Isopulegol and 1, 3, 8-P-menthatriene decreased significantly. The highest percentages of Trans-carveol (21. 85), Isoborneol (12. 90), Carvacrol acetate (11. 78) were observed in the salinity of 80mM in combined substrate of arable soil+Compost+ vermiCompost. The highest percentage of Citronellol (11. 12) and γ-Terpinene (9. 87) were recorded under Compost substrate. In the control and with a combined substrate of arable soil+Compost, the highest percentage of compounds, including 1, 3, 8, P-menthatriene (9. 93) was observed. Finally, the combined substrate of arable soil+Compost+vermiCompost caused the highest percentages of Isopulegol (8. 98) and γ-3-Carene (8. 47). Application of HA could increase the main constituents, in lemon balm.

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APA: Copy

Rahmanian, Shima, ABOUTALEBI JAHROMI, ABDOLHOSSEIN, & HOSSEINI FARAHI, MEHDI. (2022). The Effect of Culture Medium, Organic Matter and Salinity on the Amount and Active Ingredients of Lemon Balm (Melissa officinalis L. ) Essential Oil. SUSTAINABLE AGRICULTURAL SCIENCE RESEARCH, 1(4 ), 45-63. SID. https://sid.ir/paper/972976/en

Vancouver: Copy

Rahmanian Shima, ABOUTALEBI JAHROMI ABDOLHOSSEIN, HOSSEINI FARAHI MEHDI. The Effect of Culture Medium, Organic Matter and Salinity on the Amount and Active Ingredients of Lemon Balm (Melissa officinalis L. ) Essential Oil. SUSTAINABLE AGRICULTURAL SCIENCE RESEARCH[Internet]. 2022;1(4 ):45-63. Available from: https://sid.ir/paper/972976/en

IEEE: Copy

Shima Rahmanian, ABDOLHOSSEIN ABOUTALEBI JAHROMI, and MEHDI HOSSEINI FARAHI, “The Effect of Culture Medium, Organic Matter and Salinity on the Amount and Active Ingredients of Lemon Balm (Melissa officinalis L. ) Essential Oil,” SUSTAINABLE AGRICULTURAL SCIENCE RESEARCH, vol. 1, no. 4 , pp. 45–63, 2022, [Online]. Available: https://sid.ir/paper/972976/en

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