مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

165
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

0
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Molecular typing of β,-lactamase-producing Escherichia coli: Multiple Locus variable number tandem repeat analysis Versus Pulsed-field gel electrophoresis

Pages

  360-368

Abstract

 Background & Objectives: β,-lactamase-producing E. coli is the most important agent causing urinary tract infections both in community and hospitals. Strain typing including MLVA and PFGE are the most common epidemiologically tools not only for detecting the cross transmission of nosocomial pathogens but also for determining the source of infection. Materials and methods: The present study was carried of for comparison of discriminatory power of MLVA and PFGE methods. A Total of 230 isolates of E. coli from patients with urinary tract infections were examined for identifying and antimicrobial susceptibility testing MLVA and PFGE methods were used for molecular typing of all isolates. Results: Out of 230 isolates, 130 ( 56. 5%) β,-lactamase-producing E. coli isolates were found in this study. The diversity indices of the VNTR loci showed an average diversity of 0. 48 and 0. 54 for 7-loci and 10-loci respectively. The discriminatory power of PFGE showed a value of 0. 87. Conclusion: Our study showed that PFGE is more discriminatory than MVLA. MLVA is a PCR-based method and generates unmistakable data, in contrast to PFGE. Optimization of polymorphic VNTR is essential to improve the discriminatory power of MLVA basis on geographical region.

Multimedia

  • No record.

    • Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    Dolatyar Dehkharghani, Alireza, HAGHIGHAT, SETAREH, RAHNAMAYE FARZAMI, MARJAN, & RAHBAR, MOHAMMAD. (2021). Molecular typing of β,-lactamase-producing Escherichia coli: Multiple Locus variable number tandem repeat analysis Versus Pulsed-field gel electrophoresis. JOURNAL OF MICROBIAL WORLD, 13(4 (45) ), 360-368. SID. https://sid.ir/paper/999447/en

    Vancouver: Copy

    Dolatyar Dehkharghani Alireza, HAGHIGHAT SETAREH, RAHNAMAYE FARZAMI MARJAN, RAHBAR MOHAMMAD. Molecular typing of β,-lactamase-producing Escherichia coli: Multiple Locus variable number tandem repeat analysis Versus Pulsed-field gel electrophoresis. JOURNAL OF MICROBIAL WORLD[Internet]. 2021;13(4 (45) ):360-368. Available from: https://sid.ir/paper/999447/en

    IEEE: Copy

    Alireza Dolatyar Dehkharghani, SETAREH HAGHIGHAT, MARJAN RAHNAMAYE FARZAMI, and MOHAMMAD RAHBAR, “Molecular typing of β,-lactamase-producing Escherichia coli: Multiple Locus variable number tandem repeat analysis Versus Pulsed-field gel electrophoresis,” JOURNAL OF MICROBIAL WORLD, vol. 13, no. 4 (45) , pp. 360–368, 2021, [Online]. Available: https://sid.ir/paper/999447/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button