In order to evaluate of soil physicochemical characteristics by using seasonal flood at Tangestan research station in Boushehr province, 3 sites among of 10 sites (1200 ha) were selected. Two treatments (with and without water spreading) in each site were applied and 30 soil samples from 0-25 cm depth in each treatment were taken. Soil samples were analyzed for texture, bulk density, particle density, saturation percentage, total porosity, electrical conductivity, pH, organic matter, total nitrogen, calcium carbonate equivalent, solution cations and anions and sodium absorption ratio. The Factorial design with 2 factors (water spreading and site), was selected as the statistical design and the results were compared, by Duncan' s test. The final results showed that the soil texture has become heavier about one class; sand was decreased, silt and clay were increased due to floodwater spreading (significantly at 1%). The changes of soluble ions, total nitrogen and organic matter showed an increase without statistical significance. Total porosity, pH, and SAR amounts were not significant.