Human resource in extension system is considered as a key factor in agriculture and rural development process. Adequate number of well-trained staff plays a vital role for a progressive extension service.Not only, working context, financial setting, structure and management would also directly contribute in success of an efficient system, but also human resources deserves as a foundation for extension activities. The aim of this paper was to compare various working aspects of extension agents in former Ministries of Jihad-e-Sazandegi and Agriculture to screen and adopt the most desirable status for extension system in new Ministry of Jihad-e-Agriculture. The survey, however, rests on a statistic society composed of all agricultural extension education experts at national scale. Findings showed that, the extension workers have confronted with important challenges in terms of qualitative or quantitative dimensions to meet the requirements of the extension-training interventions, education degrees and capabilities for the agents, deserving people-oriented morale, and self-reliance and confidence. This deficit had a great influence on performance of extension system in Iran. Extension agents of Ministry of Jihad were significantly different regarding to: making better communication with farmers, ability to make diversity in extension programs, civility, self-confidence and self-reliance comparing with Agricultural Ministry extension workers. Results showed that Ministry of Jihad experts had got higher means in above abilities. Accordingly, the analysis revealed that extension workers of the Ministry of Agriculture also recorded statistically significant different and higher means than their co-partners in Jihad, particularly regarding to: showing higher capacities in better mutual linkage between extension and research, wider acquaintance with technical-special issues in agriculture field, being much familiar with current regulations and guidelines, and adopting qualified recording, reporting and documentation modes in operations.