Background and Aim: Heavy metals including mercury (Hg), cadmium (Cd), and arsenic(As) are toxic. long-term exposure of living organisms to them will have many risks. Although breast milk is the best nutrition for infants, it can be a source of exposure to toxicmetals. The objective of this study was to determine the levels of mercury (Hg), cadmium(Cd), and arsenic (As) in the breast milk and to investigate the effect of some parameters ontheir concentration as well as to evaluate the correlation between toxic metals and infantgrowth. Material and Method: Breast milk samples (20-30 ml) were collected from 100 mothers ataround 2nd month postpartum. Concentrations of Hg, Cd, and As were analyzed usingInductively Coupled Plasma Mass Spectroscopy (ICP-MS). The Spearman correlationcoefficient was used to determine the relationship between the concentration of the toxicmetals in milk and the defined variables. Results: Mean Hg concentration was 3. 48 μ g/L. concentration of Cd and As was in the rangeof Limit of Detection (0. 1 and 0. 2 μ g/L, respectively). In 49% of samples, the concentrationof Hg was higher than the limit recommended by World Health Organization (WHO) (1. 4-1. 7μ g/l). Mean weekly intake of Hg, Cd and As was 3. 4, 0. 098, and 0. 2 μ g/kg per week, respectively. The results showed that there was no significant relationship between Hgconcentration in breast milk and infant factors (weight, length, and head circumference ofinfants at 2nd month of age) (p>0. 05). There was a significant relationship between theconcentration of mercury in breast milk and parameters such as fish consumption andmother's occupation (p<0. 05). Conclusion: Mean concentration of mercury in breast milk samples is higher than therecommended limit. To reduce the amount of toxic metals in breast milk, preventive practice, including the holding of an educational course, should be carried out. In order to evaluate theexposure of mothers during pregnancy and lactation, monitoring programs and measuring thelevels of toxic metal in breast milk are needed in a larger statistical population.