Background and aims: The effective human resources are the main factor for realization, resuming and success of organizational purposes. In the organization milieu, human is exposed to different kinds of mental and psychological stress and pressure. In fact, stress associates with many physical and mental diseases, or has a role on making, development and mutation of them and it decreases general health of staffs, innovation and creativity abilities and also staffs' efforts. For this reason, studying stress is important regarding personnel health, psychological hygiene, and personnel performance. Therefore, main purpose of this study is to investigate the effect of job stress on general health and job performance on Air Traffic Controllers (ATC).Methods: This study is a cross-sectional, descriptive-correlation study. Statistical research community included all workers of ATC in Tehran, and then 103 individuals were selected as sample using stratified random sampling method. Flip L.R. (1992) questionnaire used for gathering data in job stress section. To collect data for general health section we used Goldberg & Hillier questionnaire and Paterson questionnaire for job performance section. The software SPSS v.19 with multi regression and simultaneously Baron and Kenny (1986) quadruplet process was used for analyzing the data.Results: The results showed that there is a negative and significant relationship between the stress which is produced in workplace and job performance. This means that increased job stress reduces job performance. Also general health has a mediating role between workplace stress and job performance.Conclusion: The results confirm the negative impact of stress on job performance and the mediating role of general health between stress and job performance in ATC. Regarding to betterment of the personal performance, it is an essential thing to design and to implement a comprehensive program in order to manage staffs' stress, to improve general health, as well as to handle the promotion and occupational safety and to accrete their participation necessities in decision-making, and these should be prioritized.