Introduction: Hirsutism is a common problem in young women that may be, sign of internal glands diseases (eg: ovary, adrenal, hypophysis,...) The aim of this research was measurement of serum level of hormones that produce by these glands in women with hirsutism.Materials and Methods: In this cross-sectional, descriptive research, in all the hirsute women that referred to Dermatology clinic of Jahrom from April 2004 to April 2005 serum level of Testosterone, Dehydroepiandrosterone-sulphate (DHEA-S), prolactin, Luteinizing hormone (LH) and Follicle-Stimulating Hormone (FSH) was measured with ELISA method.Testosterone> 1.2 ng/ml. DHEA-S > 41 mg/ml, prolactin> 25 ng/ml and LH/FSH>3 was considered abnormal. Data was analysed by chi-square method.Results: Out of 81 patients who was referred, 59 patients (72.8%) had positive FHx of hirsutism and 42 pts (52%) had irregular menstruation. Serum testosterone, DHEA-S and prolactin was high in 9.9% (7pts of 71 pts), 12.7% (10 pts of 79pts), 10.1% (8pts of 79pts) respectively and 23.8% (19pts of 80 pts) had LH/FSH>3. High level of serum testosterone DHEA-S and prolactin didn't have significant correlation with irregular menstruation (P=.429, .087, .713 respectively), Positive FHx (P= .67, .452, 1.000 respectively) and marital status (P=.684, .73, .707 respectively). LH/FSH>3 had significant correlation with irregular mensturation (P<0.001), But didn't have significant correlation with age (P=0.126), merrital status (p=.784) and positive FHx (P=.648).Overall34pts (42/5%) had at least one abnormality in their laboratory examination (testosteron or DHEA-S or prolactinor LH/FSH >3). Conclusion: Out of81 pts, 59 pts (72.8%) had positives FHx of hirsutism so it seems that genetic has significant role for inducing of hirsutism. Manypts with hirsutism [34pts (42.5%)] had at least one hormonal abnormality so as screening tests, measurement of hormones such as testosterone, DHEA-S, prolactin, LH and FSH is necessary in pts with hirsutism.